326 APPENDIX 3 



Colour the sections on each slide with one dye only, not two in 

 succession or simultaneously. Any of the following dyes may be 

 used for the times stated : — 



basic dyes 



basic fuchsine, 0-5% aq., i min. 



toluidine blue, 0-5% aq., 5 min. 



methylene blue, 1% aq., 15 min. 

 basic dye-lakes 



Ehrlich's haematoxylin, 20 min. (Alkalinize, but do not 

 differentiate.) 



Mayer's carmalum, i hr. 

 acid dyes 



acid fuchsine, 0-5% aq., 5 min. 



eosin, o-i% aq., i min. 



For a small experiment choose toluidine blue and acid fuchsine 

 only. 



Dehydrate and pass through xylene into Canada balsam or 

 similar mountant. 



Study the depth of colour in the cytoplasm and chromatin of 

 the spermatogonia and spermatocytes, and in the convoluted 

 tubules of the kidney-cortex. 



