Contents 



Preface page vii 



List of Illustrations xiii 



PART i: FIXATION 



1 Introduction to Fixation 19 



2 The Reactions of Fixatives with Proteins, i. The Visible 



Effects 31 



3 The Reactions of Fixatives with Proteins. 2. The Chemi- 



cal Changes 44 



4 The Reactions of Fixatives with Tissues and Cells: 



Methods of Research 66 



5 Primary Fixatives Considered Separately, i . Coagulants 89 



6 Primary Fixatives Considered Separately. 2. Non- 



coagulants III 



7 Fixative Mixtures 139 



PART II: DYEING 



8 Introduction to the Chemical Composition of Dyes 155 



9 The Classification of Dyes 169 



10 The Direct Attachment of Dyes to Tissues 187 



1 1 The Indirect Attachment of Dyes to Tissues 207 



12 The Differential Action of Dyes 228 



13 Metachromasy 243 



14 The Blood Dyes 262 



15 Introduction to Vital Colouring 274 



16 The Mode of Action of Vital Dyes 284 



17 A Comparison between Dyeing and other Processes of 



Colouring 296 



xi 



