Electron Microscopy for Control of Preparation of Cellular Constituents 



125 



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Abb. 1-2. Diinnschnitte durch Mitochondrien von Paniiiicciiuii caudutiiiii. Elcktroncnoptisch 

 EndvergroBeriing 49 000 bzw. 85 000 x . 



I 300 bzw. 22 500 • 



Die routinemaBige Anwendung der Kontrastie- 

 rung hat sich bereits bei ausgedehnten cytologischen 

 Untersuchungen an Protozoen (4. 9) und an Siiu- 

 gerzellen (8) gut bewiihrt. Bei der Priiparation laBt 

 sich eine Kontrastierung cytologischer Objekte mit 

 PWS Oder Tl leicht in die Normaltechnik einfiigen: 

 4*^ 2%ige isotonische gepufferte Osmiumtetr- 

 oxydlosung (bei einem dem jeweiUgen 

 Objekt adiiquaten pH-Wert) 

 l*" Tyrode-Losung 

 14-16^ 70%iger Alkohol 



\^ PWS Oder Tl, zu 1 "„ /// 70%igem Alkohol 



gelost 

 2'^ 90"oiger Alkohol 

 \^ 96%iger Alkohol 

 1^ absoluter Alkohol 

 Einbettung 



LiTERATUR 



1. Bahr, G. F., Exptl. Cell Research 5, 551 (1953). 



2. Bahr, G. F. und Muberger, G., Exptl. Cell Research 



6, 506 (1954). 



3. Hall, C. E., /. Biophys. Biochem. Cytol. 1, 1, 1 (1955). 



4. Lamb, W. G.P., Stlart-Wfbb.J.. Bill, I. G., Dovev, R., 



und Danielli, J. F., E.\ptl. Cell Research 4, 159 

 (1953). 



5. Palade, G. E., J. Exptl. Med. 95, 285 (1952). 



6. Richards, A. G., J. Appl. Phys. 23, I, 158 (1952). 



7. Sjosirand, F. S., J. Cell. Comp. Physiol. 42. 15 (1953). 



8. Wlissenfels, N., First European Regional Conf. on 



Electron Microscopy, Stockholm 1956. 



9. WoHLKARHi-Boi LERMANN, K. E.,Z. .\atur/orsch. (\957 , 



im Druck). 



The Use of the Electron Microscope to Control Preparation 



of Cellular Constituents 



M. S. C. BiRBECK and E. H. Mfrchr 



Chester Beatty Research Instiiiitc, liisiiiute of Cancer Research: 

 Royal Cancer Hospital, London, S. W. 3 



roR a number of years biochemists have investi- 

 gated cell fractions separated by centrifugation. The 

 morphological identity of these fractions has some- 

 times been determined by light microscopy, although 

 the fractions are usually characterised by their 



better control of the separation. It is also possible 

 to improve the method of separation, and some of 

 the general methods together with particular meth- 

 ods for nuclei and mitochondria which we have 

 evolved in our laboratorv, will be described. 



biochemical activity. By observing these fractions General methods. — The conventional method of 



with the electron microscope, using the sectioning separating the cell fractions uses an angle-head 



method, and by comparing them with the cell com- centrifuge; with this method fractions must be 



ponents in whole tissue, it is possible to have a washed several times by resuspcnsion and resedi- 



