132 



F, GUBA, G. HAJOSSI-KEREK AND G. ROMHANYI 



Fig. I. Chondroitin sulphuric acid. Control sample. 



Fig. 2. Chondroitin sulphuric acid-trypaflavin complex. 

 The reticular structure characteristic of trypaflavin complexes 

 can be seen very well. 



when mixed with the dye. The dyes always brought 

 about precipitation. The consistency of the precipi- 

 tates was different with every compound; the most 

 compact precipitate was obtained with rivanol. 



The results show that the change in structure is, 

 in principle, characteristic for the dye, but in addi- 

 tion the chemical composition of the substrate is 

 of importance. These conclusions were supported 

 by our polarized light analysis. 



We think that the structure which develops is 

 due to a coupling of the macromolecules through 

 the dye molecules. This is supported by the following 

 observation: we solved the newly developed structure 

 by the known ditTusion method with NaOH and 

 ethyl alcohol respectively. On the elimination of 

 either of the components the newly developed 

 structure breaks up. 



The coupling depends on the steric behaviour 

 both of the polyelectrolyte and the dye molecule. It 

 is obvious that the development of this structure 

 depends on the side groups of the acridine, chiefly of 

 its NH., groups. 



References 



1. DoGSON, K. S., Rose, F. A., and Spencer, B., Biochein. J. 



60, 346 (1955). 



2. RoMHANYi, G., Congress of the Hungarian Pathological 



Soc, Debrecen, 1955. 



