Changes in ihc Ciliary Epithelium 



139 



-Endoplosma 



VKl^ 



Ektoplasma - 



Fig. 2. Schematische Darstellung der Entstehung (A), der 

 Vermelirung (B) und der Abscheidung (C) geformter Sekrete 

 der Mitochondrien von Paramecium caiidati/m. 



drien eine Regeneration der Tubuli verfolgen, und 

 zwar bilden sich in diesen entleerten Mitochon- 

 drien zunachst wie in den Promitochondrien Mi- 

 krotubuli, die wieder zu normalen Tubuli mito- 

 chondriales heranwachsen. 



Wir haben hier also eine echte Regeneration ein- 

 zelner Mitochondrien vor uns. 

 Die Ergebnisse iiber Entstehung, Vermehrung und 

 einen Funktionsmechanismus der Mitochondrien 

 lassen sich schematisch wie in Fig. 2 zusammen- 

 fassen. Ein Abdruck der Belegaufnahmen war im 

 Rahmen dieses Tagungsberichtes nicht moglich. 

 Ihre VerolTentlichung erfolgt an anderer Stelle. 



Die Untersuchungen wurden durch cine Sachbcihilfe 

 der Deutschen F-orschungsgemcinschafl ermoglicht. Frl. 

 Chr. Brodt dankc ich wiederum fur wcrtvolle technische 

 Assistenz. 



LiTF.RATUR 



1. Ai.TMANN, H. W. u. a.. Das Cytoplasma. In Handbuch 



der Allgemcinen Palhologie, II, 1. Berlin (Goltingcn)- 

 Heidelberg, 1955. 



2. Dannfel, R. und GGttes, E., Naturwissenschaften 38, 



5, 117 (1951). 



3. EiCHENBERGER, M., E.xptl. Cell Research 4, 275 (1953). 



4. Frey-Wyssling, A., Protopiasmatologia II, A 2. Wien, 



1955. 



5. LiNonrRG, O. und Ernsti r, L., Protopiasmatologia III, 



A4. Wien, 1954. 



6. Palade, G. E., /. Histocheni. Cytodiem. 1, 188 (1953). 



7. Powers, E. L., Emret, C. F., und Roth, L. E., Biol. 



Bull. 108, 2, 182 (1955). 



8. Rhodin, J., Correlation of Ultrastructural Organization 



and Function in Normal and Experimentally Changed 

 Proximal Convoluted Tubule Cells of the Mouse 

 Kidney. Karolinska Institutct, Stockholm, 1954. 



9. Sedar, a. W. und Portir, K. R., /. Biophys. Biochem. 



Cylol. 1, 583 (1955). 



10. Sjostrand, F. S., Nature 171, 30 (1953). 



11. — International Union of Biological Sciences, Series 



B 21. Groningen, 1954. 



12. Sjostrand, F. S. and Rhodin, J., Expil. Cell Research 



4, 426 (1953). 



13. Steiner, M., Naturwissenschaften 41, H. 8, 191 (1954). 



14. Wohlfarth-Bottermann, K. E., Z. Naturforsch. (1957, 



im Druck). 



15. — • Diese Proceedings, S. 124. 



Changes in the UUrastructure of the Ciliary Epithelium during 

 Inhibition of the Secretion of Aqueous Humour 



A. Holmberg 



The Lahonitory for Biological Ultraslruclurc Research of tite Department of Aiuiloniy, Kaioliiisi\a Inslitiilet, Sloeklwlni 



It has been shown by many investigators (I, 2, 4, 

 6, 7, 8, 9, 10, 11) that the epithelium of the ciliary 

 body plays an important role in the formation of 

 aqueous humour, partly as a barrier between blood 

 and aqueous humour, and partly as an actively secret- 

 ing epithelium. This investigation coinprises only the 

 innermost, non-pigmented layer of the ciliary epithe- 

 lium. 



Rabbits were used as experimental animals. The tissue 

 has been fixed in vivo by injection of 1 "„ osmic acid in 

 veronal-acetate buffer into the posterior chamber of the 

 eye. The animals were kept under general anestesia 

 (nembutal). After 15-20 minutes part of the ciliary body 

 was removed and the fixation continued in \itri> for 

 further Zl-A hours. The animals, treated with Diamox, 

 were partly nephrectomized, partly not. The nephrccto- 

 mized animals were given 10 mg Diamox per kg body 

 weight intravenously, the others 100 mg Diamox per kg 



body weight. As control animals, besides the normal ones, 

 animals were used which instead of Diamox were given 

 Diazil in equal amounts. The fixation was carried out 

 after different intervals after the injection. 



The normal stnietiire. — In this report brief men- 

 tion will be made of some of the cell components 

 which seem to undergo changes after inhibition of 

 the secretion. 



Most characteristic for the non-pigmented epithe- 

 lium of the ciliary body arc the ;'>-cytoniemhranes. (hg. 

 1). They stand out as iriplc-laycred membranes with 

 two osmiophilic components, each about 40 A thick, 

 and one central osmiophobic layer about 70 A thick. 

 At the cell surface one can clearly see how the 

 membranes are continuous with the cell membrane. 

 Thus the /^-cytomcmbranes may be interpreted as 

 folds of the cell membrane, but it seems probable 



