Changes in l/.c Ciliary Ep it helium 



141 



Fig. 2. Golgi apparatus from a normal animal. One Golgi unit with several membranes, a tew large vacuoles and some 

 small vesicles. Magnification 53,000. 



Table 1 . The vacuolization of the Golgi apparatus 

 after Diamox. 



Number 



Experiment 



Number of Golgi Number of vacu- 

 of regions oles per Golgi 



'mals observed region per section 

 per cell 



ani 



Normal animals; no 12 



nephrectomy 

 Control animals after 3 



nephrectomy 



(Diazil) 

 30 min. after 10 mg 3 



Diamox per kg body 



weight; nephrectomy 

 60 min. after 10 mg 3 



Diamox per kg body 



weight; nephrectomy 

 120 min. after 10 mg 3 



Diamox per kg body 



weight 

 30 min. after 100 mg 3 



Diamox per kg body 



weight; no nephrectomy 



31 



78±10 



86 r 4 



198±16 



160±12 



104 ■ i: 



189: 



now, there are no other changes in the mitochondria, 

 but the analysis is not yet finished. 



In the Golgi apparatus there is a great increase in 

 the amount of the small vesicles (fig. 3). A quantita- 

 tive analysis has been performed by counting all 

 vesicles seen in the Golgi apparatus on one section 

 through a cell. The result is presented in Table 1. 

 This informs of the fact that the changes in the Golgi 

 apparatus appear very soon and that the number of 

 vesicles decreases in animals killed at longer intervals 

 after the injection of Diamox. Thus, after two hours 

 the amount of vesicles is about the same as in the 

 control animals. 



Another change in the Golgi apparatus concerns 

 the Golgi membranes, which normallv lie in 2-3 

 rows with 4-5 membrane pairs in each, sometimes 

 split up into several units with onl\ 1 3 mem- 

 brane pairs (fig. 3). 



Finally it must be pointed out that in the control 

 animals no such variations have been observed in the 

 cytoplasm, the mitochondria or in the Golgi appara- 

 tus. 



Discussion. — Concerning the cell structure under 

 normal conditions it should onl\ be emphasized 

 that the organisation of the apical parts of the cells, 

 with the ;J-cytomembranes and the basement mem- 



