STANDARDIZATION OF STAINS 



XXIX 



Alizarin red S 



Anilin blue, water soluble 



Auramine O 



Azocarmine G 



Azure A 



Azure B 



Bismarck brown Y 



Brilliant cresyl blue 



Brilliant green 



Carmine 



Chlorazol black E 



Congo red 



Cresyl violet 



Crystal violet 



Eosin, bluish 



Eosin, yellowish 



Erythrosin B 



Ethyl eosin 



Fast green FCF 



Fuchsin, acid 



Fuchsin, basic 



Giemsa stain 



Hematoxylin 



Indigo carmine 



Janus green B 



Jenner's stain 



Light green, S.F., yellowish 

 Malachite green 

 Martius yellow 

 Methyl green 

 Methyl orange 

 Methyl violet 2B 

 Methylene blue chloride 

 Methylene blue thiocyanate 

 Methylene violet 

 Neutral red 

 Nigrosin 

 Nile blue A 

 Orange G 

 Orange II 

 Orcein 

 Phloxine 

 Pyronin P 

 Resazurin 

 Rose bengal 

 Safranin O 

 Sudan III 

 Sudan IV 

 Sudan black B 



Tetrachrome stain (MacNeal) 

 Thionin 



Toluidine blue O 

 Wright's stain 



putting the stain on the market. One of these companies puts on the market 

 every stain now on the certification list. Two other companies submit samples 

 of over half the stains thus listed, while the other companies merely request 

 certification of one or two dyes in which they speciaUze. No dyes have yet 

 been certified by the Stain Commission submitted by any foreign concern 

 except for one located in Montreal. Cooperation among the Americas is 

 increasing (Conn, H. J., Stain Techn., 1942, 17, 5-6). 



In several recent editions of the National Formulary, published by the Ameri- 

 can Pharmaceutical Association, a section has been included in which formulae 

 of staining solutions are given. Originally there was no agreement between 

 these formulae and the ones recommended by the Stain Commission. Begin- 

 ning in 1937, however, it was decided that the National Formulary Committee 

 and the Biological Stain Commission should cooperate in this matter. Accord- 

 ingly, the chairman of the latter was made a member of the former and a member 

 of the National Formulary was put on the Board of Trustees of the Commission. 

 This interlocking membership is assurance that the work of preparing staining 

 formulae for each edition of the National Formulary is carried on in close coopera- 

 tion with the Stain Commission. This cooperation has resulted in two important 

 steps : 



1. Specifications of the most important stains now on the certification basis 

 have been published in the National Formulary (1942, 1946). These specifica- 



