AMIDONOPHTHOL GR 



10 



AMYL ACETATE 



aldehyde has been bound. Incubate 

 these sections in 0.5% tyramine hydro- 

 chloride in M/15 phosphate buffer pH 

 7.2 at 37°C. 24 hrs. Incubate other 

 control sections in same solution minus 

 tyramine. Immerse both test and con- 

 trol sections in Feulgen agent. Ex- 

 amine when quickly produced blue 

 color seems to be at maximum. Foci 

 of enzymatic activity, blue in con- 

 venient contrast with reddish purple of 

 "plasmal" (see Aldehydes). 



Amidonaphthol GR, see Azophloxine GA. 



Amino Acids, see Alloxan Reaction, also 

 Schmidt, C. L. A., The Chemistry of 

 the Amino Acids and Proteins. Spring- 

 field, Charles C Thomas, 1938, 1031 pp. 

 See paper chromatography added by 

 Roberts. 



Aminoacridines, some are strong antiseptics, 

 do not stain skin (Albert, A. and 

 Ritchie, B., J. Soc. Chem. Ind., 1941, 

 60, 120). 



Amitosis is direct nuclear division by con- 

 striction without formation of a chro- 

 matin thread. No special technique 

 required. Study of embryonic mem- 

 branes and of bladder of mouse (Dogiel, 

 A. S., Arch. f. Mikr. Anat., 1890, 35, 

 389-406) is suggested. 



Ammonia Carmine (Ranvier). A suspen- 

 sion of carmine in water, with slight 

 excess ammonia, is allowed to evaporate 

 in air. If it putrefies so much the 

 better. Dissolve the dry deposit in 

 aq. dest. and filter (Lee, p. 145). 



Ammoniacal Silver for branchioles — Written 

 by C. C. Macklin, Dept. of Histological 

 Research, The University of Western 

 Ontario, London, Canada. November 

 28, 1951 — This is useful to demonstrate 

 the two types of epithelium of the finest 

 bronchioles, as in the albino mouse. 

 There are two stock solutions. Solu- 

 tion 1: Five grams of silver nitrate are 

 dissolved in 300 ccms. of distilled water 

 and dilute aqua ammoniae is added 

 until the precipitate is nearly redis- 

 solved; filter; add water to make 500 

 ccms. Solution 2: One gram of silver 

 nitrate is dissolved in a small quantity 

 of water and poured into half a liter of 

 boiling water. Rochelle salt (0.83 gm.) 

 is dissolved in a small quantity of water 

 and added to the boiling solution, which 

 is then boiled for half an hour till a gray 

 precipitate gathers at the bottom of the 

 flask. Filter hot; add water to make 

 500 ccms. In using, equal parts of the 

 two solutions are mixed. The separate 

 solutions, kept in the dark, remain good 

 for a month or two. 



The etherized animals are exsangui- 

 nated, the lungs collapsed by carefully 

 nicking the diaphragm and filled via 

 the trachea with the ammoniacal silver 



solution. At the end of two minutes 

 the lungs are emptied, refilled with 10% 

 formalin, and the trachea is tied. The 

 material is placed in 10% formalin for 

 a day or more. Frozen, paraffin, or 

 celloidin sections are cut and exposed 

 to direct sunlight or weak photographic 

 developer. Flattened frozen sections 

 are useful in affording surface views of 

 lengths of bronchiolar epithelium. The 

 following description is from C. C. 

 Macklin (Canad. Jour, of Research, D, 

 1949, 27, 50-58— Bibliography) : "Two 

 definite types of cells, dark and light, 

 are revealed in the terminal bronchiolar 

 epithelium by this supravital silveriza- 

 tion. The dark cells are ciliated and 

 scattered among the light unciliated 

 cells as singles, pairs, triads or larger 

 groups to form a striking and charac- 

 teristic surface pattern. The cuticles 

 of the dark cells, far outreached by the 

 neighboring light cells, appear en face 

 as dense crowds of small uniform golden 

 brown granules. Viewed laterally these 

 show as pairs in vertical alignment, and 

 make two layers with a thin lighter band 

 between. The sides and bases are in- 

 dicated by deposits of black grains. 

 Near the sharply marked margin of the 

 definitive epithelium the dark cells are 

 shorter, more dispersed, and formed 

 like truncated pyramids with narrow 

 densely impregnated apices on some of 

 which are single delicate points instead 

 of discrete cilia. The light cells have 

 bulging villuslike free ends and make 

 flutings on the contiguous dark cells. 

 Small silverized particles sparsely 

 stipple their air surfaces, and rounded 

 plaques crown their summits. A simi- 

 lar dicellular picture is found in the 

 terminal bronchiolar epithelium of the 

 golden hamster." 



Ammonium Molybdate, as mordant for 

 Mann's stain and Weigert-Pal (Perdrau, 

 J. R., J. Path. & Bact., 1939, 48, 609- 

 610. Recommended by Bethe as a 

 fixative for supravital methylene blue, 

 see Lillie, p. 245. 



Amoeba, see Entameba. 



Amphinucleolus (G. amphi on both sides). 

 A nucleolus which is double consisting of 

 both acidophilic and basophilic parts, 

 the former is usually a central core ana 

 the latter plastered on its surface. 



Amphioxus, as an ancestor of vertebrates 

 of interest to all, see the technique of 

 Conklin, E. G., J. Morph., 1932, 54, 69. 



Amphophilic, see Staining. 



Amy! Acetate, as solvent for imbedding 

 tissues (Barron, D. H., Anat. Rec, 

 1934, 59, No. 1 and Suppl., 1-3); as a 

 clearing agent for embryological material 

 (Drury, H. F., Stain Techn., 1941, 

 16, 21-22). 



