BACTERIA. MEDIA 



32 



BALSAM 



Cystine blood agar (for P. tularensis) . 

 To 1000 cc. beef or veal infusion broth 

 add 15 gm. agar, 10 gm. peptone and 

 5 gm. sodium chloride. Autoclave 

 15 lbs., 15 min. Before use add 1 gm. 

 cystine (or cystine hydrochloride) and 

 10 gm. glucose. Dissolve by heating in 

 Arnold and sterilize 30 min. Cool to 

 50°C. add 50 cc' sterile horse blood, 

 tube aseptically in 10 cc. lots, slant and 

 incubate to prove sterility. 



Noguchi's leptospira medium. Com- 

 bine sterile 80°C. 0.9% aq. sodium 

 chloride, 100 cc. fresh rabbit serum, 

 100 cc. 2% aq. agar (melted pH 7.4) and 

 10-20 cc. rabbit hemoglobin (1 part 

 blood, 3 parts aq. dest.). Tube asepti- 

 cally in 10 cc. lots. Incubate to prove 

 sterility. 



Tryptone glucose extract milk agar. 

 Combine 15 gm. agar, 3 gm. beef ex- 

 tract, 5 gm. tryptone, 1 gm. glucose and 

 1000 cc. aq. dest. by boiling over free 

 flame. Make up volume lost with aq. 

 dest., adjust to pH 7, add 10 cc. skim 

 milk, place measured volumes in flasks 

 or tubes and autoclave 15 lbs., 15 min. 



Tellurite (for C. diphtheriae). Melt 

 infusion agar, or 0.2% dextrose agar 

 and cool to 50°C. To each 10 cc. add 

 1 cc. citrated, or defibrinated, blood 

 -f 1 cc. sterile 2% aq. potassium tellu- 

 rite, mix and pour into Petri dishes. 



Bismuth sulfite agar (Wilson and 

 Blair for E. iyphosa). Mix 20 gm. agar, 

 5 gm. beef extract and 10 gm. peptone 

 in sufficient hot aq. dest. to make 

 1000 cc. Dissolve by autoclaving 15 

 min. Store in refrigerator. (A). Dis- 

 solve 6 gms. bismuth ammonium citrate 

 scales in 50 cc. boiling aq. dest. (1), 20 

 gm. anhydrous sodium sulfite in 100 cc. 

 boiling aq. dest. (2), and 10 gms. dex- 

 trose in 50 cc. boiling aq. dest. (3). 

 Mix 1 and 2, boil and add 10 gms. an- 

 hydrous disodium phosphate while 

 boiling. Cool and add 3. Add water 

 to restore lost weight. Store in closely 

 stoppered pyrex container in dark at 

 room temperature (B). Dissolve 1 gm. 

 ferric citrate in 100 cc. aq. dest. using 

 heat and add 12.5 cc. 1% aq. brilliant 

 green. Store likewise in pyrex vessel 

 in dark. With 1000 cc. hot (A) thor- 

 oughlj' mix 200 cc. (B) and 45 cc. (C). 

 Immediatel}' pour into porous-top petri 

 dishes each 15-20 cc. After 2 hrs. at 

 room temperature store in refrigerator 

 and use within 4 days. 



Chocolate agar (for Neisseria). 

 Grind strips lean meat of 5-6 beef 

 hearts. To each 500 gm. add 1000 cc. 

 tap water, infuse in refrigerator over 

 night, strain and press through course 

 gauze. Add 10 gm. proteose peptone 

 No. 3 (Difco) per liter, heat to 50°C. 



1 hr. and boil 10 min. Strain through 

 gauze, dissolve 5 gm. sodium chloride 

 per liter and titrate to pH 7.6. Boil 

 lightly 10 min. pour off measured quan- 

 tities in flasks, autoclave 15 lbs., 15 

 min. Cool to 60°C., add 5% human or 

 horse blood, heat slowly on water bath 

 to 80-85''C. rotating to get even mix- 

 ture. Cool to 55°C. and plate. 



Bacterial Pigments. These cannot be meas- 

 ured microscopically but a method has 

 been devised for doing so with spectro- 

 photometer and photoelectric colorim- 

 eter (Stahly, G. L., Sesler, C. L. and 

 Erode, W. R., J. Bact., 1942, 43, 

 149-154). 



Bacterial Polysaccharides. Solutions of 

 reduced bases and leuco bases of penta- 

 and hexa-methyl triamino-triphenyl- 

 methane and tetramethyl diamino- 

 triphenylmethane and certain other 

 triphenylmethanes react with staphylo- 

 coccal polysaccharides and may be 

 useful in their detection (Chapman, 

 G. H. and Lieb, C. W., Stain Techn., 

 1937, 12, 15-20). 



Bacteriophage Localization by Electron 

 Microscopy, see Hennessen, W., Zeit. 

 f. wis. Mikr., 1951, 60, 172-180. 



Bacteriostatic Titration of Dyes. (Reed, 

 M. V. and Genung, E. F., Stain Techn., 

 1934, 9, 117-128). 



Bacterium Monocytogenes. Intravenous 

 injections of this organism in rabbits 

 produce a marked increase in the num- 

 ber of circulating monocytes and there- 

 fore provide an important experimental 

 method (Murray, E. G. D., Webb, R. H. 

 and Swan, M. B. R., J. Path, and Bact., 

 1926, 29, 407-439). 



Bacterium Tularense in sections. Add 10 

 cc. sat. aq. nile blue sulphate and 6 cc. 

 l%aq. safranin to60 cc.aq. dest. Stain 

 sections over night. Wash quickly, 

 dehydrate in alcohols, clear in xylol 

 and mount (Foshay, L., J. Lab. & Clin. 

 Med., 1931, 17, 193-195). 



Balances. Ordinary balances need no de- 

 scription but for weighing very small 

 amounts special balances are essential. 

 See review of literature by Gorbach, 

 G., Mikrochemie, 1936, 20, 254-336. The 

 torsion balances of Roller-Smith Co., 

 Bethlehem, Pa., are sensitive to ap- 

 proximately 2 Atgm. The quartz fiber 

 balances are still more sensitive. See 

 Click, pp. 189-191. 



Balantidium. Celloidin embedding and sec- 

 tioning (Scott, M. J., J. Morph. & 

 Physiol., 1927, 49, 417). 



Balsam for mounting sections is usually 

 satisfactory as purchased. To make, 

 mix equal parts dry balsam and sodium 

 bicarbonate and grind in mortar. Add 

 sufficient xylol to make clear solution. 



