BARBER AND KOMP 



33 



BASOPHILE LEUCOCYTE 



After few days filter and heat gently 

 (avoiding flame) to bring to suitable 

 consistency. The best mounting me- 

 dium when neutrality is essential is 

 Clarite or the cedar oil used for oil 

 immersion objectives. The latter sets 

 more slowly than balsam and it is ordi- 

 narily not necessary to employ it. See 

 Mounting Media. 



Barber and Komp thick film for malaria 

 Plasmodia (Barber, M. A. and Komp, 

 W. H. W., Pub. Health Rep., 1929, 44, 

 2330) is described by Craig, p. 290-291 

 as the most used and satisfactory of the 

 thick film techniques. His account of 

 the method abbreviated: Place large 

 drop blood on clean slide and smear with 

 needle over area about half size of that 

 usually covered by a thin blood smear. 

 Dry in incubator at 37°C., 1-1| hrs. 

 Stain in 1 part good Giemsa and 6 parts 

 neutral or slightly alkaline aq. dest., 

 about 30 min. Partly decolorize in 

 aq. dest. 5 min. (If films have back- 

 ground deep blue and leucocytes almost 

 black they may be worthless; but leav- 

 ing in aq. dest. longer may help). 

 Drain thoroughly, dry and examine. 



Barium, spectrographic analysis of, in retina 

 (Scott, G. H. and Canaga, B., Jr., Proc. 

 Soc. E.xp. Biol. & Med., 1940, 44, 555- 

 556). Barium chloride and formalin are 

 advised as fi.xative for Bile Components. 

 Barium sulphate emulsion injections are 

 recommended by Woollard, H. H. and 

 Weddell, G., J. Anat., 1934-35, 69, 25-37 

 to demonstrate arterial vascular 

 patterns. The emulsion should be of 

 such consistency that it cannot easily be 

 forced beyond the small arterioles by a 

 pressure of 1 .5 atmospheres. Fi.x tissues 

 by hypodermic injection of formalin 

 and subsequent immersion in it. Take 

 x-ray photographs of the radiopaque 

 barium. See Cretin's Test for Calcium 

 and barium under Calcium 5. 



Bartholomew, see Gram Stains Mechanism. 



Bartonella, Try Giemsa's Stain for sections. 



Basal Bodies of cilia (Wallace, H. M., 

 Science, 1931, 74, 369-370). Fix in 

 Zenker (containing acetic) or in Zenker- 

 formalin (90 cc. Zenkers + 10 cc. 10% 

 formalin). Mount paraffin sections 5/x 

 thick. After very light staining with 

 hematoxylin and thorough washing in 

 tap water dip in 0.5% aq. eosin 

 (Grubler's wasserlich. If not available, 

 use Eosin Y.) ^ min. and wash quickly 

 in large volumes of water. Make up 

 stain by adding 9 parts sat. aq. methyl 

 violet (Grubler's 6B only. If not 

 available, use CC. which is 2B.) to 1 

 part abs. alcohol 33 cc. ; aniline oil 9 cc. 

 -f methyl violet in excess. Stain is 

 best 3-8 days after mixing but the two 

 solutions can be kept separately. After 



staining sections for 2 hrs. wash well in 

 tap water, treat with Lugol's iodine 

 10-15 min. and repeat the washing. 

 Blot with filter paper. Differentiate in 1 

 part aniline oil -f 2 parts xylol. Wash 

 in several changes of xylol and mount in 

 balsam. Basal bodies deep purple, 

 nuclei dark blue. Good also for intra- 

 cellular bacteria and fibrin. 



Basement Membranes of epithelia. Suggest 

 Mallory-Heidenhain's connective tissue 

 stain as modified by Schleicher, E. M., 

 Am. J. Clin. Path.", 1943, 7, 3&^39 and 

 Schiff Reagent method as detailed by 

 McManus, J. F., Am. J. Path., 1948, 

 24, 643. 



Basic Brown, G, GX, or GXP, see Bismark 

 Brown Y. 



Basic Dyes, see Staining. 



Basic Fuchsin — anilin red, basic rubin, and 

 magenta (CI 676 or 677) — Commission 

 Certified. The tri-amino tri-phenyl 

 methane dyes bearing this name are 

 mixtures of pararosanilin, rosanilin and 

 magenta II in varying proportions. 

 They are employed for a great many 

 purposes. Basic fuchsin in a cytologi- 

 cal technique for anterior pituitary is 

 described bj' Faire, W. R., and Wolfe, 

 J. M., Anat. Rec, 1944, 90, 311-314. 

 New fuchsin (CI 678) is a different com- 

 pound. It is the deepest in color of 4 

 dyes and pararosanilin is the lightest. 



Basic Lead Acetate used as fixative for 

 Tissue Basophiles. 



Basic Rubin, see Basic Fuchsin. 



Basket Cell Arborizations, technique for, 

 Cajal, S. R., Trav. Lab. Rech. Biol. 

 Univ. de Madrid, 1926, 24, 217. 



Basophila Erythroblasts, see Erythrocytes, 

 developmental series. 



Basophile Leucocyte (mast-leucocyte, blood 

 mast cell). Least numerous granular 

 leucocyte ; percentage about 0-1 ; 

 slightly smaller (8-10m) than other 

 types; nucleus spherical or slightly 

 lobated, faintly staining and centrally 

 placed; specific granules only slightly 

 refractile, basophilic, large, variable and 

 less numerous than in other types; 

 function unknown. This cell is difficult 

 to study in fresh preparations of pe- 

 ripheral blood because it is so scarce. 

 Smears colored by the usual methods 

 (Giemsa, Wright, etc.) are satisfactory. 

 The basophilic granules appear to be 

 particularly soluble in water. Doan 

 and Reinhart (C. A. and 11. L., Am. J. 

 Clin. Path., 1941, 11, Tech. Suppl. 5, 

 1-39, with beautiful colored plates) 

 recommend supravital staining with 

 neutral red and janus green. There is 

 difference of opinion as to whether the 

 oxidase and peroxidase reactions are 

 positive (Michels, N. A. in Downey's 



