BASOPHILIC 



34 



BERBERIAN'S METHOD 



Hematology, 1938, 1, 235-372). See 

 Tissue Basophiles. 



Basophilic, see Staining. 



Bauer Negative and positive substances, 

 for method see Glycogen. 



Beams, see Ultracentrifuges. 



Bell's Method for fixing and staining of fats 

 as described by the Bensleys (p. 114). 

 Intracellular fats are mobilized by heat 

 to form droplets which are chromated 

 and later stained. Consequently the 

 preparations show these fats, in addition 

 to other microscopically visible fat, but 

 not their true distribution in the cells. 

 Fix for 10 days at 45-50 °C. in 10% aq. 

 potassium bichromate 100 cc. + 5 cc. 

 acetic acid. Imbed and make paraffin 

 sections as usual. Pass them down to 

 absolute alcohol. Stain with freshly 

 prepared Sudan III 10 min. Rinse off 

 in 50% alcohol and pass to water to arrest 

 action of alcohol. Counter-stain with 

 Delafield's Hematoxylin. Wash in 

 water, differentiate in acid alcohol, wash 

 in water again and mount in Glycerine 

 Jelly. 



Benda's Method of crystal violet and 

 alizarin for mitochondria. Fix in Flem- 

 ming's fluid 8 days (see Flemming's 

 Fluid). Wash in water 1 hr. Then 

 half pyroligneous acid and 1% chromic 

 acid, 24 hrs. 2% potassium bichromate, 

 24 hrs. Wash in running water 24 hrs. 

 Dehydrate, clear, imbed in paraffin and 

 cut sections at 4ju. Pass down to water 

 and mordant in 4% iron alum 24 hrs. 

 Stain amber-colored sol. sodium sulpha- 

 lizarinate made by adding sat. ale. sol. 

 to water, 24 hrs. Blot with filter paper 

 and color in equal parts crystal violet 

 sol. and aq. dest. (The sol. consists of 

 sat. crystal violet in 70% ale. 1 part, 

 ale. 1 part and anilin water 2 parts.) 

 Warm until vapor arises and allow to 

 cool 5 min. Blot and immerse in 30% 

 acetic acid 1 min. Blot, plunge in abs. 

 ale. until but little more stain is ex- 

 tracted, clear in xylol and mount in 

 balsam. The mitochondria are stained 

 deep violet in a rose background. The 

 colors are more lasting than in Altmann 

 preparations. This is one of the classi- 

 cal techniques of histology but it is 

 difficult. For colored illustrations see 

 Duesberg, J., Arch. f. Zellforsch., 1910, 

 4, 602-671. 



Benda's stain for fat necrosis. See Fisch- 

 ler's modification. 



Bensley's Neutral Safranin. For mitochon- 

 dria and secretion antecedents especially 

 in the pancreas. Fix in 2.5% aq. potas- 

 sium bichromate, 100 cc; mercuric 

 chloride, 5 gms. 24 hrs. Wash, dehy- 

 drate, clear, imbed and section. To 

 prepare stain slowly add sat. aq. acid 

 violet to sat. aq. safranin in a flask 



until ppt. ceases when a drop of mixture 

 on filter paper gives not an outside red 

 rim of safranin but a solid neutral color. 

 Filter. The filtrate should be as nearly 

 as possible colorless. Dry ppt. on filter 

 paper and make of it a sat. sol. in abso- 

 lute alcohol. Pass sections through 2 

 changes each of toluol and absolute 

 alcohol, then down through lower alco- 

 hols to aq. dest. (Bleach chrome and 

 osmium fixed tissues in permanganate 

 and oxalic acid, as described under 

 Anilin Fuchsin Methyl Green and 

 mercury fixed ones in Lugol's solution, 

 10 sec. finally washing in aq. dest.) 

 Dilute alcoholic neutral safranin with 

 equal volume aq. dest. and stain 5min.- 

 2 hrs. Quickly blot with filter paper. 

 Plunge into acetone and immediately 

 pass to toluol without draining. Exa- 

 mine and if further differentiation is 

 needed treat with oil of cloves. If this 

 is not enough rinse in abs. ale, flood 

 momentarily with 95% ale. and pass 

 back through absolute to toluol. Wash 

 in 2 changes toluol and mount in balsam. 

 This is a difficult method but the results 

 are worth it. (see Bensley, R. R., 

 Am. J. Anat., 1911, 12, 297-388). 



Benzamine Blue 3B, see Trypan Blue. 



Benzene-Azo-a-Naphthylamine. A mono- 

 azo dye used by Carter, J. S., J. Exp. 

 Zool., 1933, 65, 159-179 as a vital stain 

 for Stenostomum. 



Benzo Blue SB, see Trypan Blue. 



Benzo New Blue 2B, see Dianil Blue 2R. 



Benzo Sky Blue, see Niagara Blue 4B. 



Benzoazurine G (CI, 502), a direct dis-azo 

 dye of light fastness 4 sometimes 

 polychromatic (nuclei red, cytoplasm 

 blue to blue-violet). Applied after 

 treating blue-green algae with copper 

 sulphate, spores orange red, vegetative 

 cells dark blue or violet (Emig, p. 41). 



Benzene ring compounds distinguished from 

 pyrrols by Nitro Reaction. 



Benzoin Blue R, see Azo Blue. 



Benzopurpurin 4B (CI, 448) — cotton red, 

 diamin red, dianil red. Sultan and 

 direct red, all 4B — An acid dis-azo dye 

 no longer used. 



Benzoyl Perioxide treatment is recom- 

 mended by McClung, Microscopical 

 Technique, 1950, p. 71 for revival of 

 staining capacity of old smears and 

 spreads. 



Benzyl Benzoate is employed in the Spalte- 

 holz Method of clearing. 



Benzyl Violet. Conn (p. 132) states that 

 this term relates to a group of violets 

 which are pararosanilins, some acid and 

 some basic, with benzyl substitution in 

 one or more amino groups. 



Berberian's Method. Berberian, D. A., 

 Arch. Dermat. & Syphil., 1937, 36, 1171- 

 1175, has developed a method for stain- 



