HEART 



153 



HEMATOCRIT 



alum in 200 cc. aq. dest. the latter with 

 the aid of heat. Mix the 2 solutions, 

 bring quickly to boiling and add 0.5 gm. 

 mercuric oxide. Solution turns purple. 

 Cool quickly in cold water bath. Mal- 

 lory (p. 72) recommends adding 5% of 

 acetic acid. 



Heart, see Coronary Arteries, Myocardium. 

 Pericardium, Purkinje Cells and 

 Fibers. Technique and results of 

 "electron histology" of the heart are 

 presented by Kisch, B. and Bardet, 

 J. M., E.xp. Med. & Surg., 1951, 9, 1-47. 



Heart Failure Cells— Written by C. C. 

 Macklin, Dept. of Histological Re- 

 search, The University of Western 

 Ontario, Loudon, Canada. November 

 28, 1951. — This inapt designation, used 

 by pathologists, applies usually to 

 modified human pneumonocytes which 

 have become free in the lung alveoli, or 

 which are affixed to the moving mucous 

 sheet of the air tract in transit to the 

 mouth region, or which have been 

 hawked up in the sputum. The cells 

 may thus be examined in fresh mounts 

 with or without vital or supravital 

 staining; or in stained dried smears. 

 They may also be fixed, embedded and 

 cut into thin sections (Macklin, C. C, 

 The Lancet, Feb. 24, 1951, 432-435). 

 See Dust Cells. 



Heavy Water is water in which deuterium, 

 the heavy hydrogen isotope H^, has 

 taken the place of ordinary hydrogen. 

 See Deuterium which is used as a tracer 

 substance. 



Heidenhain's Azan Stain (Heidenhain, M., 

 Ztschr. f. vyiss. Mikr., 1915, 32, 361-372). 

 The following details are from Lee (1928, 

 p. 279) : Color sections 1 hr. at 55°C. 

 in 2% aq. azocarmine plus 10 drops 

 glacial acetic acid in small staining jar. 

 Wash in water. Differentiate in 96% 

 ale. 100 cc. plus anilin oil 0.1 cc. until 

 cytoplasm becomes pale pink and nuclei 

 clear red. To hurry differentiation add 

 2 drops anilin oil. Rinse in 96% ale. 

 containing few drops acetic. Put in 

 5% aq. phosphotungstic acid about 2 

 hrs. until connective tissue is com- 

 pletely decolorized. Wash rapidly in 

 water. Stain ^-3 hrs. in following solu- 

 tion diluted with equal or double parts 

 aq. dest.: anilin blue (water sol. Griib- 

 ler) 0.5 gm.; orange G, 2 gm.; acetic 

 acid, 8 cc; aq. dest. 100 cc. Examine 

 staining under microscope. Wash in 

 water, dehydrate in abs. ale, clear in 

 xylol and mount in balsam. This is a 

 very useful stain. See also McGregor, 

 L., Ajn. J. Path., 1929, 5, 545-557 for use 

 of this technique particularly as applied 

 to normal renal glomerules. Under 

 Islets of Langerhans is given use of a 



slightly modified azan method by 

 Gomori. 



Heidenhain's Iron Hematoxylin, see Iron 

 Hematoxylin. 



Heinz Bodies. These spherical bodies are 

 sometimes seen in erythrocytes espe- 

 cially when examined in the dark field or 

 when colored with Azur 1. They have 

 been referred to as Substantia Meta- 

 chromatica Granularis and B-substance. 

 The best way to demonstrate them is 

 to use the technique of Figge, F. 11. J., 

 Anat. Rec, 1946, 94, 17. Give 0.3% aq. 

 sulfanilamide to mice as drinking water. 

 Within 4-6 days these bodies will appear 

 in at least 90% of erythrocytes whence 

 they are cast out into the plasma. 

 They are most readily seen in unstained, 

 unmounted blood smears. They dis- 

 appear when studied in oil, balsam or 

 other mounting media. Heinz bodies 

 are granules of heme-containing pro- 

 tein denatured by this drug within the 

 cells. They are not produced by sod- 

 ium sulfathiazole. The Heinz Body 

 phenomenon in erythrocytes is dis- 

 cussed in detail by Webster, S. H., 

 Blood, 1949, 4, 479-504. 



Helianthin, see Methyl Orange. 



Heliozoa, techniques for, see McClung, 

 Microscopical Technique, 1950, p. 468, 

 also Rumjantzew, A., and Wermel, E. 

 Arch. f. Protistenk., 1925, 52, 217. 



Heliotrope B, see Amethyst Violet. 



Helium, see Atomic Weights. 



Kelly's Fluid is Zenker's fluid in which 5% 

 formalin is substituted for 5% acetic 

 acid. 



Helminthosporia. Stain for nuclei in (Par- 

 ris. G. K., Phytopathology, 1944, 34, 

 700). 



Helvetia Blue, see Methyl Blue. 



Hemalum (Mayer's) Hematin, 1 gra.; 90% 

 ale, 50 cc. ; aq. dest., 1000 cc. ; ammonia 

 alum, 50 gms.; thymol, 1 crystal. 

 Keeps better after adding 20 cc. glacial 

 acetic acid and making Acid Hemalum. 

 A good nuclear stain when diluted with 

 aq. dest. 1:20. The above formula has 

 been modified by Lillie (R. D., Stain 

 Techn., 1942, 17, 89-90): hematoxylin, 

 5 gm.; sodium iodate (NalOs), 1 gm.; 

 ammonia alum (A1NH4(S04)2 + 12 

 IIjO), 50gm.;aq. dest., 700 cc, glycerol, 

 300 cc, glacial acetic acid, 20 cc. No 

 ripening is necessary. Stain sections 

 formalin fixed material, 2-5 min. Blue 

 2-10 min. in tap water. Counterstain 

 in 0.2% aq. eosin Y. Dehydrate clear 

 and mount as usual. This method is 

 quick and gives a sharp stain. 



Hematin, identified by luminescence with 

 Luminol. Do not confuse with hema- 

 tein, see Hematoxylin. 



Hematocrit, a tube used to concentrate red 



