HEMOCYTOBLASTS 



155 



HERMANN'S FLUID 



Hemocytoblasts, see Erythrocytes, develop- 

 mental series. 



Hemoflagellates on tissues may be demon- 

 strated by many methods. (McClung, 

 Microscopical Technique, 1950, p. 464). 



Hemofuscin. Mallory's fuchsin stain. Fix 

 in Zenker's fluid, alcohol or 10% forma- 

 lin. Stain nuclei in paraffin or celloidin 

 sections with Iron Hematoxylin. Wash 

 thoroughly in water. Stain 5-20 min. 

 in : basic fuchsin 0.5 gm., 95% ale. 50 cc. 

 and aq. dest. 50 cc. Wash in water. 

 Differentiate in 95% alcohol, dehydrate 

 in abs. ale, clear in xylol and mount in 

 balsam in the case of paraffin sections. 

 Celloidin sections are to be cleared in 

 terpineol or origanum oil after 95% ale. 

 Nuclei blue, hemofuscin granules bright 

 red, hemosiderin and melanin unstained 

 (Mallory, p. 136). 



Hemoglobin, histochemical test (Ralph, 

 P. H., Stain Techn., 1941, 16, 105-106). 

 Flood dried blood smear with 1% 

 benzidine in absolute methyl ale, 1 

 min. Pour off and replace with 25% 

 superoxol in 70% ethyl ale, 90 sec. 

 Wash in aq. dest., 15 sec. Dry and 

 mount in neutral balsam. Hemoglobin 

 dark brown. 



Goulliart, M. C. rend. Soc. Biol., 

 1941, 135, 1260-1262 adds to frozen sec- 

 tion or dried smear a drop from a bottle 

 containing glacial acetic acid to which 

 has been added less than a week ago a 

 few crystals of potassium iodide. After 

 about 30 min. examination with a 

 polarizing microscope shows tiny boat 

 shaped birefringent crystals of proto- 

 iodoheme which later change into 

 Teichmann crystals. 



Dunn, R. 6., Arch. Path., 1946, 41, 

 676, 677. Dissolve 1 gm. cyanol (Na- 

 tional Aniline Division, Allied Chemical 

 and Dye Corporation, 40 Rector Street, 

 New York) in 100 cc. aq. dest. Add 

 2 cc. glacial acetic acid. Boil gently 

 and blue color will disappear. Keep for 

 several weeks. Immediately before 

 using filter 10 cc. and add 2 cc. glacial 

 acetic acid and 1 cc. commercial 3% 

 hydrogen peroxide. Treat frozen or 

 paraffin sections of tissue fixed in 4% 

 formaldehyde buffered to pH 7.0 after 

 rinsing in water with this fresh cyanol 

 mixture, 3-5 min. Rinse in water, 

 counterstain with 0.1% safranin in 1% 

 glacial acetic acid. Wash in water, 

 dehydrate, clear and mount in Clarite. 

 Hemoglobin, blue; nuclei, red and cyto- 

 plasm, pink (from Click, p. 63). 



Hemoglobin Estimation is done by compar- 

 ing blood with a colored paper scale or by 

 a more accurate scale in a hemoglobinom- 

 eter. The experimental error is at 

 least 5%. Staining reactions for hemo- 



globin within cytoplasm (Kindred, J. 

 E., Stain Techn., 1935, 10, 7-20). 



Hemolysis. Methods for measuring the 

 velocity of hemolysis depend on the 

 fact that red blood cell suspensions as 

 they hemolyse become more and more 

 translucent. Techniques differ merely 

 in the ways of measuring the trans- 

 mitted light. Simple visual photom- 

 eters and photoelectric ones are de- 

 scribed by Ponder, E. Glasser's Medical 

 Physics, 605-612. The same authority 

 explains the "equilibrium methods" for 

 measuring the amount of hemolysis 

 which has taken place if the process has 

 been arrested. One of these is to count 

 the cells remaining, another to deter- 

 mine the amount of hemoglobin set 

 free, etc. 



Hemophilus Pertussis. Staining of cap- 

 sules in air dried smears with 5% aq. 

 phosphomolybodic acid. Growth on a 

 special medium is advised (Lawson, G. 

 McL., J. Lab. & Clin. Med., 1939-40, 

 25, 435-43S). 



Hemosiderin, soluble in acids and other 

 reagents used in histological technique. 

 After formalin fixation the order of 

 decreasing removal is oxalic, sulphuric, 

 nitric, formic and hydrochloric. Speeu 

 of solution is but little influenced by age 

 of pigment (Lillie, R. D., Am. J. Path., 

 1939, 15, 225-239). See Iron, Di- 

 nitrosoresorcinol method. 



To demonstrate hemosiderin micro- 

 scopically pour on deparaffinized sec- 

 tions of freshly fixed tissue 1 part of 

 fresh 2% aq. potassium ferrocyanide 

 and 3 parts 1% aq. hydrochloric acid 

 heated to 60°-80°C. Thoroughly wash 

 in several changes of water. Counter- 

 stain in 0.1-0.5% basic fuchsin in 50% 

 alcohol, 5-20 min. Wash in water. 

 Pass through 95% and abs. alcohol and 

 xylol and mount in balsam. Nuclei 

 and hemofuscin, red; hemosiderin, blue 

 (J. E. Ash in Simmons and Gentzkow, 

 p. 744). See Iron and Hemofuscin. 



Heparin. A method for the histological 

 demonstration of heparin has been de- 

 scribed by Jorpes, E., Holmgren, H. and 

 Wilander, O., Ztsch. f. mikr. anat. 

 Forsch., 1937, 42, 279-301. It is based 

 on evidence that Tissue Basophiles 

 contain this substance. See also Anti- 

 coagulants. 



Heptaldehyde. An agent said by Strong, 

 L. C, Am. J. Cancer, 1939, 35, 401-407, 

 to produce liquefaction of spontaneous 

 mammary tumors of mice. It was not 

 helpful when injected into rat lepro- 

 mata (Cowdry, E. V. and Ruangsiri, C, 

 Arch. Path., 1941, 32, 632-640). 



Hermann's Fluid. 2% osmic acid, 4 cc; 

 1% platinum chloride, 15 cc; glacial 

 aceticjflkacid. 1 cc. This resembles 



