HERRING BODIES 



156 



HOFMANN'S VIOLET 



Flemming's fluid and is a good cyto- 

 logical fixative. 



Herring Bodies, see Gushing, H., Proc. Soc. 

 Exp. Biol. & Med., 1932-33, 30, 1424- 

 1425. 



Hertzberg's Victoria Blue stain for elemen- 

 tary bodies is described by Seiffert, G., 

 Virus Diseases in Man, Animal and 

 Plant. New York: Philosophical Li- 

 rary, Inc., 1944, 332 pp. It is rather 

 like Gutstein's technique (see Ele- 

 mentary Bodies) except that Hertzberg 

 does not make up the stain with po- 

 tassium hydrate and Gutstein does not " 

 destain in 1% citric acid. 



Herxheimer's solution for staining fat : 

 scarlet red (scharlach R, sudan IV), 

 1 gm. ; 70% alcohol, 50 cc. ; acetone C.P., 

 50 cc. See Sudan IV. 



Heterophile, see Staining. 



Hexamethyl violet, see Crystal violet. 



Hexuronic Acid as antiscorbutic factor 

 (Harris, L. J., and Ray, S. N., Biochem. 

 J. 1933, 27, 58-589). 



Hickson Purple, a disazo dye, giving in aq. 

 sol. a purple color to leucocytes and a 

 red color to erythrocytes introduced by 

 H. G. Cannan (J. Roy. Micr. Soc, 1941, 

 61,88-94). 



Higgins' Ink. This was apparently first 

 used as a vital stain by George Wislocki, 

 see Foot (McClung, p. 114). Dilute 

 with equal volume sterile aq. dest. 

 Warm and inject into marginal vein of 

 rabbit's ear 5 cc. daily for 3-4 days, then 

 every 3 days as long as desired. Since 

 the carbon is relatively insoluble it is a 

 simple matter to fix, imbed, section and 

 counterstain. Smaller amounts are to 

 be used for smaller animals, see Vital 

 Stains. 



Hirudinea, see Parasites. 



Hischler's Fluid, see Golgi Apparatus. 



Hiss's Method for capsule staining, see 

 Capsule. 



Histamine, improved colorimetric method 

 for estimating (Rosenthal, S. M. and 

 Tabor, H., J. Pharmacol. & Exp. 

 Therap., 1948, 92, 425). For use in de- 

 termination of the histamine content of 

 experimental tumors, see Rosenthal, 

 S. M., J. Nat. Cancer Inst., 1949, 10, 

 89-91. 



Histiocyte, a term without value as it 

 simply indicates a "tissue cell," often 

 applied to phagocytic cells of connec- 

 tive tissue. 



Histones, see discussion of Saint-Hilaire's 

 method under Purines. 



Histoplasma Capsulatum. The halos about 

 this organism and Sporotrichum 

 schencku as seen in H & E stained tissue 

 sections suggest that they are encapsu- 

 lated. Technique for search for such 

 capsules and its failure to reveal them 

 is described by Kligman, A. M. and 



Baldridge, G. D., A. M. A. Arch. Path., 

 1951, 51, 567-574. The Hotchkiss- 

 McManus stain, as employed by Klig- 

 man, A. M. and Mescon, H., J. Bact., 

 1950, 60, 415 is considered by Kligman 

 and Baldridge to be the best suited one 

 for the demonstration of capsules. 



Historadiography is the x-ray photography 

 of tissues. By a special technique 

 Larmaque, P., Bull. d'Hist. Appl., 

 1937, 14, 1-16) rays emitted at a tension 

 of 50-100 KV having a length of 0, 12-0,2 

 A° are directed upon a section closely 

 applied to a particularly finely grained 

 emulsion. The absorption of the rays 

 by the section depends upon the density 

 of its parts. Total opacity of the tissue 

 to the rays is marked on the photo- 

 graphic negative by white, permeability 

 by black, and there are usually all grades 

 between the two. Subsequent magnifi- 

 cation of about 500 times is possible, but 

 is not advisable. Sections, not more 

 than 4 microns thick, of formalin fixed 

 tissues, are recommended. An illus- 

 trated description of the appearance of 

 epidermis, cartilage, artery wall, thjToid 

 and other tissues is provided by Tur- 

 chini (J. Bull. d'Hist. Appl., 1937, 14, 

 17-28). Historadiography may have 

 many uses in the measurement of 

 densities in different physiological states 

 and in study of the distribution of sub- 

 stances opaque to x-rays experimentally 

 introduced. In some cases great den- 

 sity may accompany high Viscosity. 



Histospectrography. This is a very valu- 

 able survey method for minerals in 

 tissues. See Policard, A., Protoplasma, 

 1933, 19, 602-629; Scott, G. H. and 

 Williams, P. S., Anat. Rec, 1935, 64, 

 107-127; Cowdry, E. V., Heimburger, 

 L. F., and Williams, P. S., Am. J. Path., 

 1936, 12, 13-29. Optic lens and cata- 

 racts have been analysed particularly 

 for iron, copper and zinc (Busnel, R. G., 

 Pillet, P. and Tillie, H., Bull. d'Hist. 

 Appl., 1938, 15, 99-109). MacCardle, 

 R. C., Engman, M. F., Jr. & Sr., Arch. 

 Dermat. and Syph., 1941, 44, 429-440 

 have employed histospectrography to 

 advantage in determination of skin 

 magnesium. See Absorption Spectra. 



Hodgkin's Disease, see Reed-Sternberg 

 Cells. 



Hofmann's Violet (CI, 679)— dahlia, iodine 

 violet, primula R water soluble, red 

 violet, violet R, RR or 4RN — Conn 

 (p. 120) says above names are applied 

 rather indiscriminately to stains varying 

 in shade from methyl violet to basic 

 fuchsin which are mixtures of methyl- 

 ated and ethylated rosanilins and 

 pararosanilins having less than 5 methyl 

 or ethyl groups. He further remarks 

 that a mixture of basic fuchsin and 



