KING'S CARBOL-THIONIN 



172 



KURLOFF BODIES 



S. E., Science, 1931, 73, 529). It is 

 significant that increase in activity of 

 the renal cortex immediately follows 

 ligation of the renal artery and that the 

 mitochondria respond by enspherula- 

 tion and fragmentation within 6 min- 

 utes. The kidney is an organ in which 

 mitochondria must be examined with 

 the utmost promptness. A delay in 

 fixation of 15 minutes at room tempera- 

 ture is sufficient to cause disturbances 

 in the mitochondrial rodlets of the 

 proximal convolution. Material from 

 human autopsies is therefore of ques- 

 tionable value. Fuller, R. H., Arch. 

 Path., 1941, 32, 556-568 could find no 

 relation in a rather large number of 

 cases studied between age, hours post- 

 mortem and cause of death (except renal 

 disease) and quantity and distribution 

 of stainable lipoid. 



The interstitial framework of the 

 kidney in both normal and pathological 

 conditions is well shown by the silver 

 methods that impregnate "reticular" 

 fibers (q.v.). The "polysaccharide" 

 content of the interstitial tissue may 

 be examined by the use of periodic acid 

 (McManus, J. F. A., Amer. J. Path., 

 1948, 24, 643. See also, Ritter, H. B. 

 and Oleson, J. J., Amer. J. Path., 1950, 

 26, 639. 



The blood vessels of kidney may be 

 injected with Neoprene (q.v.) and also 

 with radio-opaque suspensions for a 

 radiographic demonstration of micro- 

 arteriography (Barclay, A. J., Amer. J. 

 Roent., 1948, 60, 1). For a demonstra- 

 tion of the functional status of the 

 renal circulation, the fluorescent dye 

 Vasoflavine may be injected into the 

 living animal and the kidney removed 

 and viewed with ultraviolet light 

 (Moses, J. B., Emery, A. J., and 

 Schlegel, J. V., Proc. Soc. E.xp. Biol, 

 and Med., 1951, 77, 233). 



The cytoplasmic particulates of the 

 renal epithelium, (mitochondria, micro- 

 somes and droplets of absorbed protein) 

 can be isolated in sucrose suspensions 

 and examined by standard biochemical 

 procedures. Cf. Oliver, J., J. Mt. 

 Sinai Hospital, 1948, 15, 175. 



King's Carbol-Thionin stain for Nissl bodies 

 (Addison in McClung, p. 450). Stain 

 paraffin or celloidin sections, 2-3 min., 

 in sat. thionin in 1% aq. carbolic acid. 

 Then wash quickly in aq. dest., differen- 

 tiate in 95% alcohol. Pass through 

 equal parts absolute alcohol and chloro- 

 form to xylol and mount in balsam. 



Kinney's Method for staining reticulum 

 (Kinney, E. M., Arch. Path., 1928, 5, 

 283). Fix 18 hrs. in 1 gm. sodium sul- 

 phantimonate dissolved in 100 cc. 4% 

 formalin immediately before using. 



Imbed in paraffin, but more than 1 or 2 

 hrs. in xylol or cedar oil will remove the 

 dark brown stain from the reticulum. 

 Hematoxylin is contraindicated as coun- 

 terstain because it obscures the color of 

 the reticulum. Other ordinary counter- 

 stains can be used. This method works 

 well even with autopsy material. It is 

 recommended particularly for kidney 

 and pancreas. Results are sometimes 

 patchy in the spleen. 

 Kleinenberg's fixative. Saturated picric 

 acid in 2% aq. sulphuric acid. Embryos 

 and marine organisms. 

 Knee-Joint, method for investigation 

 therein of radioactive gold (Ekholm, 

 R., Acta Anat., 1951, Suppl. 15, 11, 

 75 pp.). 

 Knisely, see Quartz Rod Technique. 

 Kolatchew Fluid, see Golgi Apparatus. 

 Korfif's Fibers of dentin, see Teeth, De- 

 veloping. 

 Kossa, see his test for Calcium. 

 Krajian's Congo Stain. Elastic fibers (Kra- 

 jian, A. A., Arch. Path., 1934, 18, 378- 

 380). Fix in 10% formalin, 24 hrs. or 

 more. Cut frozen sections. Wash 

 them in tap water. Place in 2% aq. 

 aluminum chloride 5-10 min. Wash and 

 stain 10 min. in 8 cc. 4% Congo red in 

 5% aq. sodium citrate -|- 2 cc. glycerin 

 C.P. After washing in tap water trans- 

 fer to 1% aq. KI for 10 sec. agitate. 

 After again washing in tap water, stain 

 5-10 min. in:anilin blue, 1.5 gm. ; orange 

 G, 2.5 gm.; resorcinol, 3 gm.; phospho- 

 molybdic acid, 1 gm.; aq. dest., 100. 

 Wash carefully in tap water. Blot sec- 

 tions on slides. Dehydrate in absolute 

 alcohol 2 min.; clear in origanum oil; 

 pass through xylol to balsam. Elastic 

 fibers bright red, fibrin dark blue. 

 Krause's End-Bulbs. Methylene blue dem- 

 onstration of in skin of forearm (Wed- 

 dell, G., J. Anat., 1940-41, 75, 346-367). 

 See Skin. 

 Krause's Membrane. Special technique 

 for, see Dahlgren (McClung, p. 427). 

 Kronig's Cement is recommended by Bens- 

 leys (p. 41) for ringing preparations 

 mounted in glycerin jelly or glycerin : 

 7-9 parts colophonium (resin) melted 

 and stirred with 2 parts beeswax. 

 Kuff, see Nucleic Acid Dye Interactions. 

 Kurloff Bodies are cytoplasmic inclusions 

 which frequently occur in the non-gran- 

 ular leucocytes of guinea pigs. They 

 show particularly well in smears of the 

 spleen, may attain a size equal to that 

 of the nucleus and can be brilliantly 

 colored supravitally by 1:2000 brilliant 

 cresyl blue in physiological salt solution 

 (Cowdry, E. V. chapter in Rivers 'book 

 on Viruses, Baltimore, Williams & Wil- 

 kins, 1928, p. 141). 



