MYELOCYTES 



217 



NAPHTHOL GREEN B 



Myelocytes, see Leucocytes, developmental 

 series. 



Myeloidin is the term applied to the mate- 

 rial of certain spheroidal or cuboidal 

 bodies of wax-like luster present in the 

 bases of retinal pigment cells of monkeys 

 and some other animals but reported as 

 absent in man and said to resemble 

 myelin. For literature see Arey, L. B. 

 in Cowdry's Special Cytology, 1932, 

 3, 1218. 



Myocardium. Method for separation of 

 fiber bundles (Mall, F. P., Am. J. Anat. 

 11,211-266). 



Myofibrils. The best method is to fix in 

 Zenker's fluid or strong Flemming's 

 mixture and to stain with iron hema- 

 toxylin (see Dahlgren in McClung 

 p. 425). Microincineration is useful 

 for the demonstration of minerals. 



Myoglia is a fine network of fibers associated 

 wuth muscle cells well demonstrated by 

 Mallory's Connective Tissue Stain. 



Myonemes of trypanosomes, gold chloride 

 method for (Ogawa, M., Arch. f. 

 Protistenk., 1913, 29, 248). See Wen- 

 rich, D. H. and Diller, W. F. in Mc- 

 Clung's Microscopical Technique, 1950, 

 p. 464. 



Myosin is a protein, present in muscle, the 

 molecules of which are needle-shaped. 

 Cross striations of muscle are thought 

 to depend on their arrangement. In 

 the isotropic (non-birefringent) bands 

 the myosin molecules are believed to be 

 disposed at random and in the aniso- 

 tropic (birefringent) bands parallel to 

 the length of the fiber (see Bourne, p. 30). 



Myriapoda, see Parasites. 



Nadi Reagent is dimethyl-paraphenylene- 

 diamin -|- a naphthol. Indophenol 

 oxidase catalyses oxidation of nadi to 

 indophenol blue and that of parapheny- 

 lene diamin to diamin. 



Nails. These very interesting structures 

 are seldom examined microscopically 

 despite the fact that changes in them 

 may provide significant clues to the con- 

 dition of other tissues. They are chiefly 

 made up of stratum lucidum thickened 

 by much eleidin. It is a simple matter 

 to macerate cut finger or toe nails in 

 40% aq. potassium hydroxide or in con- 

 centrated sulphuric acid for a few days 

 and then to isolate the individual cells 

 some of which are nucleated. Mac- 

 Leod, J. M. H., Practical Handbook of 

 the Pathology of the Skin. London: 

 H. K. Lewis, 1903, 408 pp. gives Heller's 

 method which involves fi.xation of un- 

 gual phalanx for a few days in Muller's 

 fluid, prolonged washing^ decalcification 

 for 4-6 days in 1 part nitric acid and 3 

 parts of water followed by thorough 

 imbedding in celloidin. The sections 

 can then be stained with hematoxylin. 



gentian violet, safranin or any other of 

 a number of dyes. 



Naphthalene Pink, see Magdala Red. 



Naphthalene Red, see Magdala Red. 



Naphthamine Blue 3BX, see Trypan Blue. 



Naphthamine Brilliant Blue 2R, see Dianil 

 Blue 2r. 



Naphthol Blue Black (CI, 246). Lillie, 

 R. D., J. Tech. Methods, 1945, No. 25, 

 47 pp. has reported that this dye (NAC- 

 7080 and DuFont L 6401) gives excellent 

 staining in combination: Stain with 

 Weigert's iron hematoxylin, 6 min. 

 Wash in water and counterstain 5 niin. 

 in 3 parts 1% brilliant purpurin R (CI, 

 454) in 1% aq. acetic acid and 2 parts 

 1% azofuchsin (CI, 153) likewise in 1% 

 aq. acetic acid. Rinse in 1% aq. acetic 

 acid and stain 5 min. in 1% naphthol 

 blue black (CI, 246) in sat. aq. picric 

 acid. Rinse in 1% aq. acetic acid, 

 2 min. Dehydrate and clear in alcohol, 

 alcohol and xylol, xylol and mount in 

 clarite. Collagen, reticulum and base- 

 ment membranes, dark green; smooth 

 muscle, brown; nuclei brownish-black. 



Naphthol Blue R (CI, 909)— fast blue 3R, 

 Indian blue 2RD, Meldola's blue, new 

 blue R, phenylene blue — An oxazin dye 

 used by Harvey, B. C. H., and Bensley, 

 R. R., Biol. Bull., 1912, 23, 225-249 as a 

 supravital stain for gastric mucosa. 

 The Bensleys' report that this dye has 

 proved useful in the localization of un- 

 suspected parathyroid and thyroid tis- 

 sue in experimental animals. After 

 vascular perfusion in a concentration of 

 1 : 40 ,000 of .85% aq . sodi um chloride the 

 thyroid, parathyroid and lymph nodes 

 become colored intensely blue ; whereas 

 other tissues, muscles, salivary glands 

 etc., are colored pale greenish blue. 



Naphthol Green, see Naphthol Green B. 



Naphthol Green B (CI, 5) — acid green O, 

 green PL, naphthol green — An acid 

 nitroso dyefor whicha probable formula 

 is given by Conn (p. 42) and which he 

 thinks was the naphthol green used by v. 

 Volkmann, R. and Strauss, F., Zeit. f. 

 Wis. Mikr., 1934, 51, 244-249, and by 

 MoUier, G., Zeit. f. Wis. Mikr., 1938, 55, 

 472-473. 



Lillie, R. D., J. Techn. Methods, 1945, 

 No. 25, 47 pp. recommends naphthol 

 green B for connective tissue. Stain 

 sections 6 min. in Weigert's or other 

 iron hematoxylin. Wash thoroughly 

 in water and stain 3 min. in 1% aq. 

 eosin Y (CI, 768 . Rinse in water and 

 mordant 4 min. in 10% dilution of 

 U.S. P. ferric chloride solution. Rinse 

 in water and stain 5 min. in 1% naph- 

 thol green B. Differentiate 2 min. in 

 1% aq. acetic acid. Dehydrate in 

 aceton, clear in acetone-xylene and in 

 .xylene and mount in clarite xylene or 



