SALMONELLA 



310 



SCHULTZE'S METHOD 



elusions caused by viruses green. A 

 very brilliant stain but the green fades 

 in the course of a month or two. Stand- 

 ardized safranin O technique advised 

 by C. H. Sawyer (Stain Techn., 1940, 

 15, 3-7) is: overstain deparaffinized sec- 

 tions in 0.1% light green S.F. or fast 

 green FCF in 50% alcohol adjusted to 

 pH 2.4 with 0.1 A^ HCl (100 cc. = 20 cc. 

 0.5% stain + 50 cc. 100% alcohol + 

 8 cc. 0.1 A^ HCl + 22 cc. aq. dest.) 

 for 4 hrs. or more. Destain in Soren- 

 sen's buffer pH 8, 30 minutes or more. 

 Overstain in 0.1% aq. safranin O at 

 least 4 hrs. Rinse in aq. dest. De- 

 stain in 0.01 A^ HCl (pH 2) or in 0.001 

 N HCl (pH 3) for light green and fast 

 green respectively, 15 min. After rins- 

 ing in aq. dest. dehydrate in 2 changes 

 dioxan, pass through xylol and mount 

 in balsam. As fixatives Sawyer finds 

 Petrunkevitch's paranitrophenol-cu- 

 pric-nitrate-nitric and picro-formol-ace- 

 tic better than Bouin's fluid. Zenker's 

 fluid can be employed. 



Salmonella, Flagella of non-motile, Ed- 

 wards, P. R., Moran, A. B. and Bruner, 

 D. W., Proc. Soc. Exp. Biol. & Med., 

 1946, 62, 296-298. See Triphenyltetra- 

 zolium chloride. 



Samarium, see Atomic Weights. 



Sandarac mixed with dioxan, camphor and 

 salol is recommended by McClung 

 (p. 40) as a mounting medium in place 

 of balsam. 



Sandison's Technique for inserting trans- 

 parent chambers in rabbit ears (Sandi- 

 son, J. C, Anat. Rec, 1924, 28, 281). 

 This has been improved by Clark, E. R. 

 et al., Anat. Rec, 1930, 47, 187-211 and 

 by Abell, R. G. and Clark, E. R., Anat. 

 Rec, 1932, 53, 121-140. See modifica- 

 tions by Williams, R. G., Anat. Rec, 

 1934, 60, 487-491 and by the same 

 author (ibid, 493-499) the latter for 

 insertion into skin. Moore, R. L., 

 Anat. Rec, 1935-36, 64, 387-403) has 

 adapted the chamber for insertion into 

 dog's ear. 



Saponin, for hemolysis in centrifugal isola- 

 tion of nuclei from chicken erythro- 

 cytes (Dounce, A. L. and Lau, T. H., 

 Science, 1943, 97, 584-585). 



Sarcolemma. Special technique for, see 

 Dahlgren in McClung's Microscopical 

 Technique, 1950, p. 341. 



Sawyer, see Safranin-Iight green. 



Scandium, see Atomic Weights. 



Scarlet B or EC, see Biebrich Scarlet, 

 water soluble. 



Scarlet B Fat Soluble, see Sudan III. 



Scarlet J, JJ, V, see Eosin B or bluish. 



Scarlet R, see Ponceau 2R. 



Scarlet Red, see Sudan IV. 



Schaudinn's Fixative. Sat. mercuric chlo- 

 ride in 0.85% aq. sodium chloride 2 



parts. Add 1 part 95% ethyl alcohol 

 and enough glacial acetic to make 1% 

 solution immediately before use. For 

 Protozoa, staining in bulk. 



Scheele's Green, an exogenous pigment, 

 copper arsenite. 



SchiflF's Reaction for aldehydes (Bourne, 

 p. 22) is basis of Feulgen reaction for 

 Thymonucleic Acid. 



Schistosomes. When it is necessary to 

 collect at autopsy all parasites irrespec- 

 tive of the stage of development or 

 location, a modification of previous 

 techniques described by Pan, C, and 

 Hunter, III. G. W. (J. Lab. & Clin. 

 Med., 195, 37, 815-816) is suggested. 

 To infect small mammals with Schisto- 

 soma Japonicum see method of Pan, 

 C, Kaufman, E. H., and Hunter, III, 

 G. W., Ibid., 817-819. 



Schlesinger's Reagent. Add to 4 gms. zinc 

 acetate in a bottle 95% ethyl alcohol to 

 make up 100 cc. Shake occasionally 

 and use supernatant fluid. See Uro- 

 bilin. 



Schmitt, see Polarization Optical Method. 



Schneider's Aceto-Carmine, see Aceto- 

 Carmine. 



Schultz, H. Cholesterol Test. Cut frozen 

 sections of formol fixed material. Place 

 sections in a 2.5% solution of iron alum 

 mordanting for 3 days in low tempera- 

 ture (37°) oven. Rinse the sections 

 after removal from the alum solution 

 in aq. dest. to which are added a few 

 drops of nitric acid (2 to 3 drops per 

 26 cc). This removes alum precipitate 

 in the sections. They are then trans- 

 ferred to 2-3% gelatin solution and 

 mounted in dilute gelatin on the slide. 

 After the mounted sections have com- 

 pletely dried add a few drops of a mix- 

 ture of equal parts of concentrated 

 sulphuric acid and glacial acetic acid. 

 The appearance of a blue -green color 

 indicates that cholesterol, either in free 

 or ester form, was present in the sec- 

 tions before treatment. Both acids 

 must be of analytical reagent standard 

 and the sulphuric acid at least 98% 

 pure. The appearance of bubbles in 

 large numbers indicates impure re- 

 agents. See Knouff, R. A., Brown, 

 J. B. and Schneider, B. M., Anat. Rec, 

 1941, 79, 17-38. Revised by R. A. 

 Knouff, Dept. of Anatomy, Ohio State 

 University, Columbus, Ohio, April 24, 

 1946. Swyer, G. I. M., Cancer Re- 

 search, 1942, 2, 372-375 has checked in a 

 satisfactory way the Schultz test with 

 quantitative determinations of cho- 

 lesterol in normal and enlarged pros- 

 tates. 



Schultze's Method for clearing embryos 

 has been modified by Miller. See 

 Cartilaginous Skeleton. 



