TRIACID BLOOD STAIN 



358 



TRICHROME-STAINS 



paraffin with xylol. Pass through 2 

 changes abs. ale. Treat 1-1^ min. in 

 4% uranium nitrate in abs. ale, 20 cc; 

 25% gum mastic in abs. ale, 4(>-50 cc; 

 abs. ale, 20-30 cc. Wash in at least 3 

 changes aq. dest. until streaks of gum 

 mastic are removed. 0.1% aq. silver 

 nitrate in water bath at 100°C., 1-1§ 

 hrs. Wash in aq. dest. Then through 

 80% and 95% to abs. ale. 10-12.5% gum 

 mastic in abs. ale. 5 min. Repeat 3 

 washings described in aq. dest. Re- 

 duce 20-30 min. in: hydroquinone, 10 

 gm.; 12.5% gum mastic in abs. ale, 

 1 cc.; aq. dest., 200 cc. (with tempera- 

 ture gradually raised to 100°C.). Wash 

 thoroughly in aq. dest. Counterstain 

 with hematoxylin and eosin if desired. 

 Dehydrate in abs. Clear in xylol and 

 mount in balsam. The advantages are 

 speed and decrease in confusing silver 

 deposits. See Steiner's illustrations. 

 A technician's experience with Steiner's 

 method has been published (Wilson, 

 R. A. J., Am. J. Clin. Path., 1946, 16, 

 21-24). 



10. Nigrosine has been proposed as a 

 negative stain for treponema (Dienst, 

 R. B. and Sanderson, E. S., Am. J. 

 Public Health, 1936, 26, 910). Com- 

 parison of dark field, nigrosine stain 

 and Kahn test in diagnosis (Nagle, N., 

 J. Lab. & Clin. Med., 1939-40, 25, 660- 

 661). 



11. Ziehl's fuchsin stain (Perrin, T. 

 G., Am. J. Clin. Path., 1943, Tech. 

 Suppl., 7, 28). Make smears on slides 

 of exudate secured by compressing base 

 of chancre or by scraping surface of 

 ulcer. Dry in air and fix by heat in 

 flame, if desired. Stain 2 min. while 

 heating, or for 6 min. at room tempera- 

 ture, being careful not to let the stain 

 dry. The stain is aq. dest., 10 cc; 

 commercial formalin, 1 cc; acetic acid, 

 1 cc; Ziehl's fuchsin (Ziehl's Carbol- 

 Fuchsin) 4 cc. Wash in water, moving 

 gently, and dry in air. See Vincent's 

 Angina. 



Triacid Blood Stain, see Ehrlich's. 



Tri-Amino Tri-Phenyl Methane Dyes. 

 These are the rosanilins. Examples: 

 acid fuchsin, acid violet, anilin blue WS, 

 basic fuchsin, benzyl violet, crystal 

 violet, ethyl green, ethyl violet, Hof- 

 mann's violet, iodine green, isamine 

 blue, magenta II, methyl blue, methyl 

 green, methyl violet, new fuchsin (ma- 

 genta III), pararosanilin (magenta O), 

 rosanilin (magenta I), spirit blue, vic- 

 toria blue B and R and victoria blue 4R. 



Trichinella Spiralis. Mallory (p. 304) gives 

 as a rapid method of diagnosis the 

 squeezing of small pieces of jaw muscle 

 or of muscle near tendon of diaphragm 

 between two slides and direct examina- 



tion at low magnification. A useful 

 device for squeezing the muscle, called 

 a "trichinoscope" has been constructed 

 by Gould, S. E., Am. J. Clin. Path., 

 Teehn. Suppl., 1944, 8, 98-100. If 

 trichinellae are calcified or encapsu- 

 lated specimens can be cleared with 

 acid. In permanent preparations of 

 Zenker or formalin fixed material 

 stained with hematoxylin and phloxine 

 or eosin the parasites are best seen in 

 longitudinal sections of muscle fibers. 

 To demonstrate in migratory phase 

 withdraw blood from vein in arm into 

 syringe containing 3% aq. acetic acid, 

 centrifuge and examine. 



Rapid iodine-silver technique (Kal- 

 waryjski, M. B. E., Wojsk. Przegl. 

 Weteryn., 1938, 9, 123-136). Place thin 

 slices of muscle for 10 min. in iodine, 

 potassium iodide, aq. dest. sol. in fol- 

 lowing proportions 2:4:100 or 0.5:1:100 

 or 0.1:0.2:100 Wash in aq. dest. 

 Destain in 2.5% aq. sodium thiosulphate 

 until muscle is clear. Wash in aq. dest. 

 Equal parts 10% aq. silver nitrate and 

 strong ammonia until iodine leaves para- 

 sites. Wash in aq. dest. Decolorize 

 in 5% aq. sodium thiosulphate. Wash 

 in aq. dest. and mount in glycerin. 

 Parasites stained dark brown owing to 

 conversion of iodine to silver iodide. 



See investigation of larvae with radio- 

 active phosphorus (McCoy, O. R., 

 Downing, V. F. and Voorhis, S. N., J. 

 Parasit., 1941, 27, 53-58). 



Trichomonas Vaginalis, technique for, Van 

 Someren, V. D., Vet. J., 1946, 102, 

 73; detailed by Wenrich, D. H. and 

 Diller, W. F. on McClung's Microscop- 

 ical Technique, 1950, p. 465. 



Trichloracetic Acid employed with mercuric 

 chloride and acetic acid as a fixative 

 (Heidenhain, Zeit. wiss. Mikr., 1909, 

 25, 405) also used in 4 or 5% aq. sol. as 

 decalcifying agent. 



Trichlorethylene, as a solvent in histo- 

 logical technique in place of xylol 

 (Oltman, R. E., Stain Techn., 1935, 10, 

 23-24). 



Trichlorlactic Acid used as fixative fol- 

 lowed by staining with resorcin fuchsin 

 for cytoplasmic canalicular apparatus 

 (Holmgren, E., Ergeb. d. Anat., 1901, 

 11, 274-329; Cowdry, E. V., Internat. 

 Monatsschr. f. Anat. u. Physiol., 1912, 

 29, 1-32). 



Trichosiderm name suggested for iron pig- 

 ment from red hair (Flesch, P. and 

 Rothman, S., J. Invest. Dermat., 1945, 

 6,257-270). 



Trichrome-Stains. There are many such 

 stains. See Mallory's and Masson's. 

 A rapid one is described by Pollak, 

 O. J., Arch. Path., 1944, 37, 294. Com- 

 position of stain: acid fuchsin, 0.5 gm.; 



