EEPORT OF THE SECEETARY. 51 



retesting of cattle with tuberculin to guard against the reappearance 

 or reintroduction of the disease. The testing of dairy herds in Mary- 

 land and Virginia which suppl}' milk to the city of Washington 

 has also been continued. During the fiscal year the tuberculin test 

 was applied to 4,327 cattle in Virginia, 1,847 in Maryland, and 1,967 

 in the District of Columbia. The percentage of diseased cattle among 

 those not previously tested was 16.06, while in the retests it was only 

 3.95. Seventv-three reacting animals in the District of Columbia 

 were slaughtered, and in all but one case the lesions of tuberculosis 

 were found on post-mortem examination, thus verifying the result 

 of the tuberculin test. 



INSPECTION or LI\TE STOCK FOR INTERSTATE MOVEMENT. 



In addition to work already reported, the bureau inspects live stock 

 for interstate movement for purposes other than immediate slaughter, 

 and tests cattle with tuberculin and horses and mules with mallein, 

 when such measures are required by the laws of the State or Ter- 

 ritory to which the animals are destined. In this work 52,230 cattle 

 were inspected during the year, of which 18,778 were tested with 

 tuberculin. Similarly 34,789 horses and mules were inspected and 

 5,789 tested with mallein. 



DOURINE or HORSES. 



An outbreak of a disease of horses in Iowa, suspected of being 

 dourine, was reported in May, and a prompt investigation was made, 

 as a result of which the scientists of the Bureau of Animal Industry 

 were able by prolonged search with the microscope to find in the 

 blood the causative organism of the disease. This was the first time 

 that the organism had been demonstrated in a natural infection in 

 the United States, although the disease had existed in this country 

 for some years and had been stamped out about five years ago. The 

 manner in which the present outbreak was introduced was not posi- 

 tively determined, although indications pointed strongly to its hav- 

 ing been brought in by an imported stallion. Strict quarantine 

 measures were at once enforced, with the cooperation of the Iowa 

 State authorities, and the disease is now believed to be practically 

 eradicated. 



HOG CHOLERA. 



The practical value of the serum for the prevention of hog cholera, 

 produced after long experimentation by the Bureau of Animal 

 Industry, is now generally recognized. At the suggestion of the 

 department, the large hog-raising States have taken up the manu- 

 facture and distribution of the serum, and upward of 200,000 inocula- 



