BUREAU OF ANIMAL INDUSTRY. 117 



not an important site of urea formation. The results of the ex- 

 periments in the second problem taken as a whole indicate that 

 muscular tissue has considerable glycolytic properties; that is, 

 ability to cause the disappearance of carbohydrates, and that these 

 properties arc most active for a few hours folloAving the death of 

 the animal. Although carbohydrates are broken down, the process 

 does not proceed to the complete oxidation of sugar to carbon dioxid 

 and water, but rather to the formation of an intermediate product, 

 which in all likelihood is lactic acid. Papers reporting the results 

 of these investigations have been prepared for publication in a 

 scientific journal. 



TUBERCULIN AND MALLEIN. 



During the fiscal year there were furnished to various State, 

 county, and city officials 600,702 doses of tuberculin for testing cattle 

 for tuberculosis and 395,455 doses of mallein for testing horses and 

 mules for glanders. Arrangements have been made for these labora- 

 tories to supply sufficient mallein to meet all of the needs of the 

 War Department. 



Laboratory studies have been made of processes for refining and 

 concentrating tuberculin, and progress has been made in the pro- 

 duction of a tuberculin which is considerably more potent than that 

 ordinarily produced. Some of these refined products have been ap- 

 plied to the ophthalmic diagnosis of tuberculosis. 



ZOOLOGICAL DIVISION. 



B. H. Ransom, Chief. 

 ROUNDWORMS OF SHEEP. 



In experiments at the farm near Vienna, Va., leased by the bureau 

 for carrying on investigations of stomach worms and other similar 

 parasites of sheep, it was found that lambs born in the spring could 

 be carried through the summer season without material injury from 

 internal parasites if the flock was moved every week after the grazing 

 season began (about May 1) to fresh areas of ground, which had been 

 prepared for grazing by planting with rye, oats, millet, or cowpeas, 

 and if the mothers were dosed once a month with 100 cubic centi- 

 meters of 1 per cent solution of copper sulphate. The dosing of the 

 mothers was done for the purpose of destroying stomach worms, and 

 thus reducing the danger of infecting the lambs from this source. 

 After M'eaning the lambs in July they were separated from their 

 mothers, and during the remainder of the summer were kept moving 

 over fresh-planted pastures, the hurdles being changed every week. 

 In marked contrast, other lambs which were kept during the summer 

 with their mothers in a permanent pasture nearly all died and showed 

 on post-mortem examination serious infestation Avith internal para- 

 sites, particularly stomach worms. 



Sheep born in the spring of 1915 and carried through the re- 

 mainder of the year by following various methods of handling were 

 thereafter maintained in a thrifty condition by occasionally moving 

 them to fresh-planted pastures ' during the spring, summer, and 

 autumn of 1916 and dosing them once a month with 100 cubic centi- 



