THE COLLOIDAL NITROGEN IN THE URINE FROM 

 A DOG WITH A TUMOR OF THE BREAST 



MAX KAHN AND JACOB ROSENBLOOM 



(Biochetnical Laboratory of Columbia University, at the College of 

 Physicians and Surgeons, New York) 



In 1892 Töpfer^ found that the urine of patients suffering from 

 Cancer contained a very large amount of "extractive substance." 

 This " extractive substance " was calculated by first determining the 

 total nitrogen, and then subtracting from this amount the sum of the 

 nitrogen values for the urea, uric acid, and ammonia contained in 

 the same urine. Bondzynski and Gottlieb,^ five years later, reported 

 that the nitrogen in oxyproteic acid was 2 to 3 per cent, of the total 

 urinary nitrogen. Salkowski,^ and Hess and Saxl,* using different 

 procedures in their researches, came to the conclusion that the oxy- 

 proteic acid or the alcohol-precipitable substances are increased in 

 the urine of human beings suffering from Carcinoma. 



Salkowski and Kojo,^ in a preliminary communication, recently 

 suggested several methods for the determination of colloidal nitro- 

 gen in the urine. A year later Kojo published the results of a com- 

 parative study of the various procedures suggested in this connec- 

 tion.^ Einhorn, Kahn and Rosenbloom^ studied the zinc sulfate- 

 precipitable, colloidal, nitrogenous material from the urine of nor- 

 mal subjects as well as from the urine of carcinomatous patients, 

 and came to the conclusion that the amount of colloidal nitrogen 

 was invariably increased in subjects with carcinomatous growths. 



* Töpfer: Wiener klin. Wochenschrift, 1892, v, p. 49. 



'Bondzynski and Gottlieb: Zentralbl. f. d. med. Wissenschaften, 1897, xxxv, 



P- 577- 



* Salkowski : Berliner klin. Woch., 1910, xlvii, p. 1746. 



*Hess and Saxl: Beiträge zur Carcinomforschung, 1910, Part II. 

 'Salkowski and Kojo: Berl. klin. Woch., 1910, xlvii, p. 2297. 

 'Kojo: Zeitschr. f. physiol. Chem., 191 1, Ixxiii, p. 416. 



^Einhorn, Kahn and Rosenbloom: Amer. Journ. of Gastro-enterology, 1911, 

 i, p. 2; and Archiv f. Verdauungs-Krankheiten, 191 1, xvii, p. 557. 



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