I9I2] Walter H. Eddy ' 115 



In the first of two experiments on frogs, the initial dose failed 

 to produce any strychnin effects. An effect followed the second 

 injection of an equal dose, but it required three doses to produce 

 Opisthotonus. Recovery was complete. In the second frog, each 

 of two doses injected successively produced Opisthotonus. The 

 frog recovered. 



For the first dog a double dose was required to produce hyperes- 

 thesia and tetanus. The results with the second dog duplicated 

 those with the first. 



In all these physiological tests the strychnin appeared to be 

 liberated slowly in the animal, the effects Coming on gradually and 

 extending over a period of 3-5 hours, with complete recovery. 



Concliisions regarding strychnin mucoid. The results seemed to 

 leave no doubt regarding the compound-nature of this product. 

 Whether it is a true salt or an adsorption Compound can not be de- 

 cided from the available data, but its neutrality, its water-solubility, 

 and its power to yield both strychnin and mucoid, strongly suggest 

 the production of a salt by a process directly comparable to the 

 neutralization of base by acid. 



The physiological tests show that the Compound evidently con- 

 tains a much smaller proportion of strychnin than that in the com- 

 mon Sulfate. The quantitative examinations have not yet been 

 completed. 



C. Conin mucoid. Conin combines with mucoid very rapidly 

 and yields a Solution which filters easily. The product, af ter purifica- 

 tion by dialysis and alcohol precipitation, is water-soluble and biuret- 

 reacting. As in the case of morphin mucoid, however, it was im- 

 possible to demonstrate the presence of the alkaloid. All tests 

 were negative with the potassio-mercuric iodid and phospho-tung- 

 stic acid reagents. 



D. PiPERiDiN MUCOID. The purified piperidin product gave the 

 protein tests and also a test for piperidin with platinic chlorid. 



E. Anilin mucoid(?). A water-soluble product of mucoid 

 and anilin was obtained but the anilin disappeared early in the puri- 

 fication process. 



F. Morphin nucleoprotein. Two attempts were made to 

 produce a Compound of morphin with yeast nucleoprotein. The 



