ii6 Sonic Protein Compounds [Sept. 



method of preparation was similar to that described on page 112. 

 Neither attempt was successful in establishing the presence of mor- 

 phin in the final product. A water-soluble protein of different char- 

 acter from the nucleoprotein resulted in each case. 



G. Morphin caseinogen, strychnin caseinogen, and cal- 

 cium CASEiNOGEN. Studies were made of the effects of morphin, 

 strychnin and calcium hydroxid on caseinogen. In each case water- 

 soluble, biuret-reacting products were obtained. Rigorous purifi- 

 cation was not attempted. 



Salts of ovomucoid. Neumeister^ investigated a glucoprotein 

 in eggs which he named " pseudopeptone." This Compound was 

 studied by Salkowski,^ Mörner/ and Eichholz,^ and called by them 

 " ovo-mucoid." As a " cell-protein," this substance seemed to offer 

 good material for our experiments. A pure product was prepared 

 by Mörner's''' well-known process. 



Preparation of ovo-mucoid. (From eggs.) With increasing 

 purity, precipitation with alcohol became correspondingly difficult. 

 Alcohol-ether did not remove this difficulty but the addition of a 

 few drops of sodium chlorid Solution brought about precipitation in 

 every case. The final water-solution was freed from chlorid by 

 dialysis in a parchment bag in the presence of toluene. The Solu- 

 tion, which then was acid to litmus, was evaporated to dryness at 

 40° C, yielding yellow flakes which were ground to a white powder. 



Properties of the ovo-mucoid product. This ovo-mucoid was 

 readily soluble in water and gave a good biuret test. The water- 

 solution frothed on shaking, but was not viscid. Phosphotungstic 

 acid, 0.2 per cent. hydrochloric acid, 4 per cent. acetic acid and 

 tannic acid Solutions precipitated the aqueous Solution, which was 

 acid to litmus. 



(From shad roe.) The roe was ground in a mortar with sand 

 and this mixture poured into boiling, slightly acidulated, water. 

 The remaining Steps were identical with those for the preparation of 

 ovo-mucoid from eggs and the product responded to the same tests. 



These two products were used in the following studies. 



' Neumeister : Zeitschrift für Biologie, 1890, xxvii, p. 331, 



'Salkowski: Centralhlatt f. d. med. Wissensch., 1893, xxxi, pp. 513 and 706. 



^ Mörner : Zeitschr. f. physiol. Chem., 1893, xviii, p. 525. 



' Eichholz : Journ. Physiol., 1898, xxiii, p. 163. 



