igi2] Walter H. Eddy 117 



H. Strychnin ovo-mucoid (egg). The method of prepara- 

 tion followed the lines of the morphin-mucoid process (page 112) 

 with the following abbreviation : After dialysis the Solution was at 

 once precipitated with absolute alcohol. No other methods of puri- 

 fication were used. 



The filtrate f rom the original mixture of ovo-mucoid and strych- 

 nin was turbid and acid to litmus, but became neutral on standing, 

 in the presence of toluene. On dialysis, and consequent dilution 

 with water, the Solution clarified. The dialysate on the other band 

 became turbid but failed to give a protein or strychnin test, 



When the dialyzed liquid was treated with absolute alcohol, in 

 excess, a mixed, cheesy and gelatinous precipitate was produced. 

 The alcoholic filtrate from this precipitate was acid and gave a 

 strychnin test, suggesting dissociation. The precipitate dissolved 

 readily in water and the Solution was then filtered. It was nozv acid 

 in reaction and gave no strychnin test. Precipitated again with 

 alcohol, the solid product failed to give the strychnin test, was acid 

 and resembled in every way the original ovo-mucoid. 



A portion of this precipitate was dissolved in water and the So- 

 lution evaporated to dryness at 40° C. A new trituration with 

 strychnin was made with this product. The results were the same 

 as with the first preparation, viz., a turbin Solution that cleared on 

 dilution with water by dialysis and gave in this condition both 

 strychnin and protein tests. Alcohol again dissociated it into 

 strychnin and ovo-mucoid (?). 



From the above results it was deemed desirable to make a care- 

 ful study of the reactions of the product and a second preparation 

 was conducted for this purpose. The turbid filtrate obtained from 

 the initial mixture of strychnin and ovo-mucoid was found to be 

 actually amphoteric to litmus, though acid to Phenolphthalein. Its 

 alkalinity to litmus was not increased by retriturating it with strych- 

 nin. Dilution with water resulted again in a clear Solution, giving 

 both strychnin and protein tests. On standing for a considerable time 

 in a parchment bag, in the presence of toluene, the amphoteric reac- 

 tion gradually disappeared and the Solution became distinctly acid to 

 litmus. It also finally yielded a precipitate in the bag and lost its 

 power to respond to the strychnin test. The turbid dialysate grad- 



