ii8 Sonic Protein Compounds [Sept. 



ually acquired protein material, but the frequent renewals of water 

 and large voliime made it impossible to determine the presence of 

 strychnin. Apparently complete dissociation resulted, but neither 

 the character of the dissociation products nor the manner in which 

 the strychnin separated was determined. 



Strychnin ovo-niucoid (roe). The results with ovo-mucoid 

 from shad roe were identical with those in the case of tgg ovo-mu- 

 coid except that the disappearance of the strychnin on dialysis was 

 much slower. It was ten days before the contents of the bag failed 

 to give the strychnin test. Concentration of the dialysates in this 

 case before applying the strychnin test failed to make its detection 

 possible. 



Evidence of the Compound natnre of the ovo-mucoid products. 

 The clear amphoteric Solution, with its response to strychnin and 

 protein tests, indicates a chemical combination, especially in view 

 of the water-insolubility of strychnin. The dissociability in alcohol 

 of the shad roe product, and the results of dialysis, indicate that it 

 is more stable than the strychnin product with &gg ovo-mucoid. 

 Again the question of whether we are here dealing with a true 

 chemical Compound or with an adsorption product remains open for 

 further investigation. 



2. Protein-protein Compounds. The foregoing experiments 

 were preliminary to attempts to bring about combinations between 

 acid-reacting and basic-reacting proteins, such as protamins and 

 histons. 



/. HiSTON MucoiD. Preparation of histon hydrochlorid. His- 

 ton was prepared by the method of Huiskamp.^ Thymus glands 

 from freshly killed calves were freed from fat with a knife and 

 minced in a meat chopper. The hash was then placed in a large 

 bottle and extracted in an ordinary ice box for 24-48 hours with 

 distilled water. About 300 c.c. of water were used with each 100 

 grams of thymus. The extract was filtered through wet fluted filter 

 papers. Nucleohiston was precipitated from the filtrate with 5 c.c. 

 of IG per Cent, calcium chlorid Solution per 100 c.c. of extract. The 

 precipitate was then filtered off and redissolved in water to which a 

 little ammonia had . been added. This Solution was filtered and 

 reprecipitated with calcium chlorid Solution in the usual way. The 



* Huiskamp : Zeitscjir. f. physiol. Chemie, 1901, xxxii, p. 145. 



