igi2] Walter H. Eddy 119 



precipitate was then extracted with 0.8 per cent. hydrochloric 

 acid for the production of the hydrochlorid. This extract of histon 

 hydrochlorid was finally dialyzed in a parchment bag against dis- 

 tilled water until neutral to litmus. This Solution of histon hydro- 

 chlorid was used for the preparation described below. 



Preparation of potassium mucoid. Acid-free mucoid was dis- 

 solved in 0.3 per cent. potassium hydroxid Solution and the liquid 

 filtered. The filtrate was then dialyzed in a parchment bag against 

 distilled water (in the presence of toluene) until neutral to litmus. 

 The product in this neutral Solution was presumably potassium 

 mucoid. 



Preparation of histon mucoid. Histon hydrochlorid Solution 

 was added drop by drop to the potassium mucoid Solution. A pre- 

 cipitate formed immediately, and sedimented quickly beneath the 

 clear supernatant liquid. Excess of the histon hydrochlorid Solu- 

 tion dissolved the precipitate. The product was then filtered off 

 and washed with water until the washings no longer gave precipi- 

 tates with ten per cent. ammonium hydroxid or 0.2 per cent. hydro- 

 chloric acid Solution. 



Evidence of the Compound nature of the histon mucoid product. 

 A portion of the precipitate was triturated with 0.05 per cent. so- 

 dium carbonate Solution. A colloidal Solution was obtained. Its 

 filtrate gave a heavy precipitate with 0.2 per cent. hydrochloric 

 acid Solution and a distinct precipitate with ammonium hydroxid 

 Solution. 



These results did not determine whether the sodium carbonate 

 merely dissolved the histon mucoid, or dissociated it into a histon So- 

 lution and a sodium mucoid Solution. To ascertain these points 

 the following tests were made : 



(a) A portion of the sodium carbonate Solution was poured into 

 95 per cent. alcohol. It failed to precipitate at once or on Standing. 



(b) A portion of the sodium carbonate Solution was poured into 

 95 per cent. alcohol, to which one drop of 10 per cent. sodium 

 chlorid Solution had been added. A precipitate appeared on 

 Standing. 



(c) Alcohol-ether failed to precipitate the Solution but with the 

 addition of a drop of salt Solution a precipitate appeared. 



