I9I2] Walter H. Eddy 121 



Solutions yield histon hydrochlorid and insoluble mucoid; (c) pure 

 histon mucoid and o.i per cent. potassium hydroxid Solutions yield a 

 potassium mucoid histon complex. 



The failure to get a histon precipitate with ammonia in the 

 potassium hydroxid extract may have been due to the small amount 

 of resultant histon mucoid or to the formation of an insoluble form 

 o£ histon, such as ammonia produces. Whatever the explanation of 

 this failure, there seemed to be no doubt of the power of histon 

 to combine with mucoid to form a Compound different in proper- 

 ties from either histon hydrochlorid or potassium mucoid. 



/. Histon nucleoprotein (yeast). Neutral potassium nu- 

 cleoprotein (obtained by dissolving yeast nucleoprotein in o.i per 

 cent. potassium hydroxid Solution and dialyzing free from hydroxyl 

 ions) combines with histon hydrochlorid in the same way as potas- 

 sium mucoid (page 119). Much more of the Solution of histon 

 is necessary for the production of the salt. The product was similar 

 to histon mucoid in being insoluble in water; in dissolving readily in 

 0.05 per cent. sodium carbonate Solution but incompletely in 0.5 

 per cent. sodium carbonate Solution ; and in forming, with sodium 

 carbonate, a water-soluble sodium-histon nucleoprotein complex. 



K. Histon ovo-mucoid. Preparation. The ovo-mucoid {tgg) 

 was purified to such a degree as to be practically soluble in salt-free 

 alcohol (page 116). A similarly pure Solution of histon hydro- 

 chlorid was used. 



When the water Solutions of these two substances were com- 

 bined, a precipitate formed slowly. A slight excess of the histon 

 Solution dissolved the precipitate. The precipitate dissolved to a 

 turbid Solution in 0.05 per cent. sodium carbonate Solution. This 

 turbid fluid was filtered and divided into two portions. One por- 

 tion was poured into 95 per cent. alcohol to which 3 drops of 10 

 per cent. sodium chlorid Solution had been added. The other por- 

 tion was saturated with ammonium sulfate. Both portions gave 

 heavy precipitates, which were soluble in water; the Solutions were 

 precipitated in part by ammonia. When purification of the ammo- 

 nium sulfate precipitate by dialysis was attempted, the Compound 

 broke down. The "alcohol precipitate" was hydrolyzed with 

 hydrochloric acid. The resultant liquid, neutralized with potassium 



