124 Epinephrin Effects on Metabolism [Sept. 



nection, althongh it is possible that this mode of administering 

 epinephrin has a different effect on nitrogenous metabolism, as is the 

 case in carbohydrate metabolism (Löwi).® 



IL DESCRIPTION OF THE EXPERIMENTS 

 This investigation consisted of two metabolism experiments. 

 One animal was used for both experiments. An intermediate pe- 

 riod served to allow the animal to recuperate from the effects of the 

 first experiment before the second one was begun. The metabolism 

 work was conducted by the general methods in use in this labo- 

 ratory.'^ 



We determined the nitrogen content in the several ingredients 

 of the food. Urinary nitrogen, in the leading forms, was deter- 

 mined as f ollows : ammonia, each day ; total, urea, creatin and Crea- 

 tinin (as Creatinin), every third day; purins, at the end of each 

 period. The urine was preserved with thymol. Total nitrogen was 

 determined by the Kjeldahl process ; ammonia and Creatinin by the 

 Polin methods f iirea^ by Benedict's method ;^^ purin nitrogen by a 

 combination of the Arnstein" and Salkowski^^ methods. 



The authors used two specimens of the colorless "adrenalin 

 Chlorid" (i:i,ooo) of Parke, Davis and Co. They were pur- 

 chased in the open market. Each was tested for its pressor action 

 at the conclusion of the corresponding injection experiments and 

 was then found to be practically as active as ever. Varying 

 amounts and concentrations of "adrenalin chlorid" were injected 

 into the peritoneal cavity ; in the first experiment the concentration 

 was 1 : 10,000 — in the second, i : i,ooo. In one injection period of 



*Löwi: Von Noorden's Metabolism and practica! medicine, 1907, iii, p. 1181. 



' Mead and Gies : Anier. Journ. Physiol., 1901, v, p. 106 ; also Gies and collab- 

 orators: Biochemical Researches, 1903, i, Reprint No. 21; Gies: Amer. Journ. of 

 Physiol., 1905, xiv, p. 403; Gies: Amer. Journ. of Physiol., 1901, v, p. 235; also 

 Gies and collaborators : Biochemical Researches, 1903, i, Reprint No. i ; Gies : 

 Proc. Amer. Physiol. Soc, Amer. Journ. of Physiol., 1904, x, p. 22; Hawk and 

 Gies: Amer. Journ. of Physiol., 1904, xi, p. 177. 



* Polin : Amer. Journ. of Physiol., 190S, xiii, p. 45. 



" No glycosuria occurred. Examinations were made repeatedly. 



^^ Benedict : Journ. of Biol. Chem., 1910, viii, p. 405. 



" Arnstein : Zeit. f. physiol. Chem., 1897, xxiii, p. 417. 



" Salkowski : Salkowski's Manual of physiol. chem. and path., 1904; Arch. d. 

 ges. Physiol., 1897-98, Ixix, p. 268. 



