1912] Alfred P. Lothrop 165 



13. The occurrence and estimation of Creatinin in urine/ 



Stanley R. Benedict, l'he work contemplates a thoroiigh inves- 

 tigation of the question as to whether the Jaffe reaction in urine is 

 due entirely, as is usually assumed, to the form of Creatinin which 

 is ordinarily isolated from urine, or whether other substances may 

 not be partially responsible for the reaction. The results indicate 

 that there are two (or more) forms of Creatinin in urine, both of 

 which yield the Jaffe reaction and also a zinc chlorid Compound, but 

 which differ from each other in certain specific properties. A 

 change in the ratio between these two forms of Creatinin in the 

 urine has been observed in certain abnormal conditions. The most 

 marked change was noted in inanition. There is probably a third 

 substance contributing to the Creatinin reaction of urine which is 

 in no wise related to Creatinin, but appears to be a weak acid. The 

 study is in progress. 



14. An endeavor to prepare phrenosin from protagon.^ 

 Louis E. Bisch. Thudichum's method^ of isolating phrenosin has 

 apparently never been reviewed. It was assumed that this method 

 could be applied with success directly to protagon. The author was 

 unable to do so, however. Repetitions of each of the numerous 

 Steps in the process, with as much as 1450 grams of protagon at 

 a time (in faithful accord with Thudichum's description), failed 

 to yield sufficient material with which to complete the directions. 

 It is possible that losses, which seem to have occurred at all stages of 

 the process, totally consumed any phrenosin that existed in the orig- 

 inal protagon. It is Dr. Gies' Intention to study this possibility 

 further. 



15. Mucoid-silver products.^^ Louis E. Bisch. Mo'ist, a-cid- 

 free tendomucoid, triturated with a moderate amount of moist, 

 alkali-free silver oxid, yields a brown to black mixture which be- 

 comes very viscid when a small volume of ammonium hydroxid So- 

 lution is stirred into it. A mechanical excess of 10 per cent. 

 ammonium hydroxid Solution converts the viscid mass into a brown 



^ Under the auspices of the George Crocker Special Research Fund. 



* Bisch : Dissertation (Part I), Columbia University, 1912. 



* Thudichum : A treatise on the chemical Constitution of the hrain, 1884, pp. 

 136-8. 



"Bisch: Dissertation (Part II), Columbia University, 1912. 



