I9I2] 'Alfred P. Lothrop i77 



believe that it is possible to produce death by apnea, caused in turn 

 by acapnia, without the assistance of the toxic effects of morphin 

 and ether. The toxic effects of these drugs must be included as 

 factors contributing to the shock, 



This report deals with only one of the phases of the relation of 

 acapnia to shock, namely the relation of acapnia, produced by ex- 

 cessive artificial respiration, to shock ; and as it is only a preliminary 

 report, it is not intended as an answer to Henderson's contention. 

 Its purpose is mainly to record two general f acts : (A) That we 

 have reduced the amount of carbon dioxide in the blood to nearly 

 40 per Cent, of the normal amount, and have maintained this reduc- 

 tion for a period of 3 hours, without producing Symptoms of shock; 

 and (B) that there are other factors than depletion of the störe of 

 carbon dioxid, which, unless properly guarded against, can in them- 

 selves cause the death of the animal under experimentation. 



27. Biochemical studies of sulfocyanate.^^ Max Kahn. 

 A. The ferric chlorid colorimetric test for sulfocyanate in saliva 

 is inexact and unreliable. A negative result by the Bunting suction 

 method is no evidence of the absence of sulfocyanate but a positive 

 result is suggestive of the presence of a comparatively large amount. 

 The pink color spontaneously disappears from the ethereal layer 

 in positive tests by the Bunting suction method. Various medicinal 

 substances, and also certain Compounds that result from biological 

 transformations of proteins and carbohydrates, if excreted in the 

 saliva, give a very marked red coloration in the ferric chlorid test, 

 similar to that produced by sulfocyanate. 



B. Sulfocyanate occurs in the saliva and salivary glands of man, 

 in the salivary glands of oxen, but apparently not in the salivary 

 glands of dogs. It occurs in the blood, but the spieen, the pancreas, 

 the thymus, the thyroid and the testicles of dogs do not contain it. 

 The liver seems to be the gland in the body that contains most 

 sulfocyanate, which is also present in bile and in the small intestines. 

 The stomach contents of dogs on an ordinary diet were free from 

 sulfocyanate. When, however, sodium sulfid was given, the gastric 

 mixture contained sulfocyanate. 



'^Kahn: Dissertation, Columbia University, 1912. Conducted under the 

 auspices of the Dental Society of the State of New York. 



