21 8 Differential Chemical Study of Glucoses [Jan. 



Zone with a melting point of 189-190° C, equivalent to 41 grams 

 of sugar per thousand. After hydrolysis^ of a large quantity of 

 urinary residue, the values for polariscopic deviation, reduction, and 

 fermentation were about ten tinits less than the corresponding 

 figures for non-hydrolyzed urine ; and the quantity of sugar in the 

 hydrolyzed urine, as represented by osazone, did not equal half 

 the amount of sugar obtained from the original urine. These dif- 

 ferences can be explained in several ways ; but since " diabetic 

 sugar," as I have already demonstrated, is a collection of several 

 distinct chemical substances, it is highly probable that in such treat- 

 ment some of these components are so modified that they no longer 

 form osazones, although they retain their fermentability. It is tnie 

 that this explatiation does not accord zvith conventional views, but, 

 nowadays, the phenoniena in chemistry and physics zvhich do not 

 agree zvith current theories are the ones that shoidd be probed and 

 investigated in the interest of^ triith. 



When a portion of evaporated, syrupy residue from my pa- 

 tient's urine was allowed to age, there was a marked change in the 

 residue, due either to the nature of the syrup or to chemical decom- 

 position in it. This was not surprising in view of the complex 

 composition of diabetic urine, and the further fact that the original 

 sugar may undergo a process of slow hydrolysis, or rather conden- 

 sation, to form higher Compounds like dextrins and analogous sub- 

 stances, which then yield, with Phenylhydrazin, resinous pseudoösa- 

 zones having very low melting points, such as I have isolated from 

 gastric Juices.'^ 



Discussing, now, some of the details of my analytic data, I find 

 that in the second treatment^ down to the fourth extraction, the 



' Hydrolysis was performed in the following way : 100 c.c. of urine, or 

 urinary extract, were heated for seven hours on a water bath with 5 c.c. of 

 hydrochloric acid Solution (strength not stated). The liquid was then evapo- 

 rated to a volume of 60 c.c. 



^ Landolph, Revista de la Universidad de Buenos Aires, October, November 

 and December, 1910. 



* Professor Landolph examined a number of urines from the same patient 

 by several processes for the determination of the content of sugar. The urines 

 were evaporated over a water bath to a syrupy consistency and the examina- 

 tions of the separate urinary residues are called "Treatment" I, II, etc. After 

 completion of the alcoholic extractions, a portion of each extract was hydrolyzed 



