1913] Frederic Landolph 219 



residue,^ though always quite abundant, decreased from 68.17 grams 

 per thousand parts of urine in the first to 36.26 grams in the third. 

 On comparing the figures obtained from the original urine with the 

 figures for the extracts of its residues, I observed polariscopic devia- 

 tions which were only one-fourth, one-fifth and one-tenth as great, 

 respectively, as those for the untreated urine. The disappearance of 

 the aldehydic function led me to suppose that there was condensa- 

 tion and not oxidation, since fermentation and reduction tests were 

 still very marked and showed the presence of half or even more of 

 the total dry residue. As regards the characteristic osazone, it was 

 obtained only in small amounts with nearly, but not quite, the same 

 melting point, i. e., instead of melting at 189-190° C. it melted at 

 185-186° C. ; whereas the pseudoösazone (with a melting point of 

 yS~7^° C.), which was resinous and alcohol-soluble, corresponded 

 to 66.72 grams of sugar per thousand (33.36 grams of polymer- 

 ized sugar). , 



Hydrolysis produced analogous changes in the urine, but here 

 the triic osazone, with a melting point of 174-175° C, was repre- 

 sented by the small quantity of 1.75 grams per thousand of urine 

 (0.87 gram of glucosej. This, there fore, was different from the 

 osazone obtained from the non-hydrolyzed material. The pseudo- 

 ösazone from the hydrolyzed Solution amounted to 44.94 grams of 

 sugar per thousand, or 22 units less than the pseudoösasone from 

 the non-hydrolyzed Solution (melting point of 95° C. instead of 

 75° C). For the fourth, fifth and sixth alcoholic extracts of the 

 first treatment, analogous figures were obtained. 



In the first extract^^ of the second treatment, the degree of the 

 reduction was maintained for each Observation, while the fermenta- 

 bility of the non-hydrolyzed Solution became very feeble. Here the 

 principal osazone was pseiidoösazone (with a melting point of 

 112° C.) amountingto 38 grams per thousand of urine (17.5 grams 



with dilute hydrochloric acid Solution and the " sugar s" in the hydrolyzed and 

 non-hydrolyzed portions fractionated with basic lead acetate and mercuric 

 nitrate. [Trans.] 



' After the urine was evaporated over a water bath to a syrupy consistency, 

 the residues were successively extracted with absolute alcohol. These " treat- 

 ments " are called " extraction " i, 2, etc. 



^" After extraction with alcohol, 24.167 grams of extract were dissolved in 

 500 c.c. of water. This was divided into two parts. The first part was pre- 

 cipitated with basic lead acetate, the second with mercuric nitrate. 



