220 Differential Chemical Study of Glucoses [Jan. 



of glucose). The tnic osazone, with a melting point o£ 177- 

 178° C, amounted to only 1.74 grams per thousand of iirine (0.87 

 gram of glncose). After hydrolysis^^ we obtained in three differ- 

 ent fractions (12.20, 10.62 and 2.96) a total of 25.78 grams of 

 osazones per thousand of urine (14.39 gi'ams of glucose). The 

 melting points were respectively 170°, 177° and 157-159° C. It 

 is Singular that here no pseudoösazoncs were obtained. 



For the second extract^^ of the second treatment, we obtained, 

 from the non-hydrolyzed Solution, osazone with a melting point 

 of 95~97° C., amounting to 11 grams per thousand of urine (5.5 

 grams of sugar). Here too, after hydrolysis, we obtained two 

 fractions of tnie osazone (melting points of 172-173 and 180- 

 181° C.) amounting to 19.75 grams per thousand of urine (9.87 

 grams of sugar) but again no pseudoösazone. 



For the third alcoholic extract of the second treatment, we ob- 

 tained practically no fermentation, and only psctidoösasones. In 

 the hydrolyzed Solution, however, fermentation was pronounced; 

 and we obtained a mixture of true osa:^ones (melting points of 180- 

 190° and 187-188° C.) amounting to 10.06 grams per thousand 

 of urine (5.03 grams of sugar — 5.40 grams of fermentable sugar). 

 The pseudoösazone with a melting point of 100° C. and amounting 

 to 6.83 grams per thousand of urine (3.42 grams of sugar), 

 corresponded, perhaps, to the difference between 7.42 grams of 

 sugar per thousand of urine (polariscopic determination) and 

 5.40 grams per thousand of urine (estimated by fermentation), i. e., 

 2.02 grams. 



For the fourth alcoholic extract of the second treatment, we 

 obtained from both the non-hydrolyzed and hydrolyzed Solutions, 

 mixtures of true and pscudoösazones in approximately equal pro- 

 portions. 



In the alcoholic extracts of the third treatment, fermentation in 

 general was always pronounced, corresponding to an increase in the 

 amounts of true osazones and pseudoösazones; but, with this 



" Hydrolysis was conducted by heating the extract with hydrochloric acid 

 Solution over a water bath. See footnote 6. 



*' The residue remaining after the first alcoholic extract was again ex- 

 tracted with alcohol, and also treated with mercuric nitrate and basic lead 

 acetate. See footnotes 8 and 9. 



