1913] Frederic Landolph 221 



anomaly, that in the sixth and seventh extracts (third treatment) 

 the vveights of the sugars, as represented by the amounts of pseiido- 

 ösa^ones, were three or four times greater than the weights of the 

 total dry residues. 



As regards the fourth, fifth and sixth treatments/^ we obtained 

 neither polariscopic deviation, reduction nor fermentation, but we 

 did obtain pseudoösasones. 



We also noticed that for the fifth alcoholic extract of the second 

 treatment the total dry residue obtained upon hydrolysis was always 

 greater than the total dry residue of the non-hydrolyzed portion, 

 evidently due to the absorption of oxygen from the air (which per- 

 haps also provoked a condensation or resinification of Phenylhy- 

 drazine). 



In Order to obtain an approximately correct idea of the action of 

 basic lead acetate or mercuric nitrate upon the urinary glucoses, I 

 treated each of the first and second extracts of the second treatment 

 with basic lead acetate in one portion and mercuric nitrate in an- 

 other. The lead sali precipitated nearly all of the optically active 

 siigar from the non-hydrolyzed Solution, white the mercuric nitrate 

 did not have any effect in this regard, but both reagents diminished 

 the quantity of the reducing sugars. 



From the first fraction of the second treatment, basic lead 

 acetate removed all the sugar that yielded pseudoösazones, and left 

 only the sugar which formed true osazones — the latter in dimin- 

 ished quantities, at least in the first and second crystallizations. 

 Treatment with mercuric nitrate yielded only traces of true osasones 

 but, in the second crystallization, a marked amount of pseudoösa- 

 zoncs was obtained. 



From another portion of the second alcoholic extract of the 

 second treatment, basic lead acetate again precipitated all the active 

 sugars, while mercuric nitrate did not affect them. With mercuric 

 nitrate, only pseudoösazones were obtained. 



*' Procedures similar to those previously indicated were adopted in the study 

 of " treatments " IV, V and VI : The urine was evaporated in each case, the 

 residue extracted with alcohol several times, and each of the extracts divided 

 into two portions. One portion was hydrolyzed, the other was not. The 

 hydrolyzed and non-hydrolyzed portions were individually treated in different 

 portions with basic lead acetate and mercuric nitrate. See footnote 8. 



