I9I3] 



Arthur W. Dox and Ray E. Neidig 



409 



and to the control, 25 c.c. of water. Toluene was used throughout 

 as an antiseptic. After two weeks, during which time the flasks 

 were shaken at frequent intervals, the formol-titration was made, 

 with the results given in Table i. 



Comparing the foregoing results with those obtained with hip- 

 puric acid,^ it will be noted that the extent of the cleavage of the acid 

 into its components is in this case considerably less. This can hardly 

 be taken as evidence in support of any assumption that we are 

 dealing with two separate enzymes. Quantitative differences ob- 

 served in work of this nature have little significance, unless the 

 same enzyme preparation has been employed and a uniformity of 

 all other conditions maintained. 



TABLE 2 



Data pertaining to the formation of ammonia. 



As in our previous work with hippuric acid, the contents of the 

 flasks after this titration were distilled with magnesium oxide for 

 the determination of ammonia. 



Ammonia can hardly be regarded as a direct product of the 

 cleavage of pyromucuric acid. The small amount found probably 

 results from further decomposition of the glycocoU by another 

 enzyme. This cleavage of glycocoll is, however, so slight as to be 

 practically negligible. 



Thiophenuric acid was not available at the time these experi- 

 ments were carried out. We propose to test the activity of mold 

 enzymes toward this substance at a future date. 



melted at 163° C. ; 0.25 gm. required 14.8 c.c. of n/10 Ba(0H)2 Solution for 

 neutralization ; theory, 14.8 c.c. 

 'Dox and Neidig: Loc. cit. 



