I9I31 



Walter H. Eddy 



425 



the literature is that of Lawrow (18). Ammonia is used to precipi- 

 tate the histon and purification is secured by redissolving- in liydro- 

 chloric acid Solution and reprecipitating with ammonia. It intro- 

 duces no new features. 



III. PROPERTIES OF HISTONS 



1. Results of elementary analysis. A summary of the avail- 

 able analytic data is presented in Table i. 



2. Solubilities. The solubilities of the histon products are in- 

 dicated by the data in Table 2. 



TABLE 2 



Data pertaining to the solubilities of histons 



Kind 



1. Goose-blood (12) 



2. Goose-blood (12) 



3. Goose-blood (2) 



4. Thymus 



5. Thymus 



6. Salmin (22) 



7. Arbacin (21) 



8. Globin (23) 



9. Scombron (2) 



10. Gadus (16) 



11. Lota (8) 



12. Para-histon (9) 



13. Hen-blood (i) 



14. Centrophorus and Sphäre- 



chinits (15) 



Precipitated by 



NaCl 



NH4OH 



NaOH 



NH4OH 



NaCl 



NaCl 



Alcohol 



NH4OH 



NaOH 



NaCl 



NH4OH 



Alcohol 



NH4OH 



Reagents 



S* 

 I 



s 

 I 



s 

 s 

 s 

 s 

 s 



S5 



I 



s 



I* 



I 



I 



I 



I 



I 



I 



II 



I 



I 



I 



I 



I 



ffio 

 f~) '' 



I 

 I 



s 



Si 

 Si 



Sä 

 81 

 I 

 I 



Si 



s 





X ° 



2: 



I 

 I 

 I 



I 

 I 

 I 



S3 



I 



I 



I 



I 



s 

 I 



t/1 ü 

 CO 



Si 

 Si 



s» 

 s» 



SI 

 SI 

 SI 



s 



SI 



SI 

 SI 

 S 



(No data given by Kossei). 



S 

 S 

 S 



S2 



S 

 S 



s 

 s 

 s 

 s 

 s 

 s 

 s 



S Ol 



3 



I 

 I 

 I 

 I 

 I 

 I 



I 

 I 

 I 

 I 



s 



* S = soluble ; I := insoluble. 



* Soluble in excess of the reagent. 

 "Becomes insoluble if allowed to stand. 



' Precipitate dissolves in slight excess of ammonia. 



* Alkali precipitates (30 per cent.) water Solution. 



" Kossei States properties are same as those of ordinary histon. 

 properties are assumed to be identical with those of i. 



Hence the 



