I9I3] Walter H. Eddy 437 



The precipitate was next completely dissolved in water with 

 the addition of a little 10 per cent. potassium hydroxid Solution 

 and the liquid again dialyzed free from hydroxyl ions in a parch- 

 ment bag. This neutral Solution was then reprecipitated with 

 calcium chlorid as before, yielding again a sharply separating pre- 

 cipitate and a clear filtrate which gave neither biuret reaction nor 

 alcohol precipitate. The precipitate was then washed with alcohol, 

 and ether, and dried to constant weight at 105° C. A Solution in 

 dilute potassium hydroxid Solution, dialysed free from hydroxyl 

 ions, gave characteristic protein tests. The dried product was then 

 analysed for nitrogen, ash and calcium. 



The filtrate from the calcium chlorid-precipitated product was 

 treated with a few drops of conc. hydrochloric acid Solution, yield- 

 ing a copious precipitate and a water-clear filtrate, which gave no 

 biuret test or alcohol precipitate. The precipitate was purified by 

 Solution in 0.3 per cent. potassium hydroxid Solution and reprecipi- 

 tation with hydrochloric acid; washing with water, alcohol, and 

 ether, and finally drying to constant weight at 105° C. The dry 

 product was analyzed for total nitrogen and ash. Both precipitates 

 were rieh in phosphorus. 



A. B. 



Product precipitated 

 Product precipitated with hydrochloric acid 

 with calcium chlorid, in the filtrate from A 

 Per Cent. Per Cent. 



Total N 7.65 15.97 



Ash 7.30 5.96 



Calcium None Not determined 



These results agree with Huiskamp's views in certain respects. 

 Assuming that water extracts iboth a nucleoprotein and a nucleo- 

 histon, treatment of the product with hydrochloric acid may be 

 assumed to separate the histon from the nucleohiston, and leave a 

 mixed residue of histon-free nucleoprotein and a nuclein. Both of 

 these substances dissolve in 0.3 per cent. potassium hydroxid Solu- 

 tion, but the latter is precipitable by calcium chlorid while the 

 former is not. Furthermore, the view that calcium chlorid precipi- 

 tates a nuclein rather than nucleic acid is borne out by the protein 

 reactions of the product precipitated by calcium chlorid. Huis- 

 kamp, however, claims that his nuclein fonns a calcium salt with 



