iQi 3l Walter H. Eddy 439 



Histon prepared by the salt-precipitation method offers excel- 

 lent material for the study of protein-salt formation. 



The necessity of knovving the method o£ preparation of a given 

 histon prodiict, in order to understand the properties ascri'bed to it, 

 is obvious from these sttidies. 



The quantitative data on page 434 were obtained with the assist- 

 ance of Mr. E. G. Griffin and those on page 437 with the aid of 

 Mr. W. F. Hume, to whom I am greatly indebted for the effective 

 Cooperation they have given. I wish also to acknowledge my indebt- 

 edness to Dr. Gies for facilities and suggestions throughout the 

 entire research. 



VII. SELECTED BIBLIOGRAPHY OF HISTON STUDIES 



1. Ackerman, D. Zur Chemie der Vogelblutkerne. Zeitschr. f. phys- 



iol. Ch., 1904, xliii, p. 299. Gives Plenge's method of preparing 

 histon from hen blood. 



2. Bang, Ivar. Studien ueber Histon. Z&frf.^ 1899, xxvii,p. 463. First 



report of the discovery and preparation of scombron, a histon in 

 mackerei sperm. Also presents comparisons with previously dis- 

 covered histons, a discussion of the ammonia reaction, and first 

 list of the " five characteristic properties " of histon. 



3. . Bemerkungen ueber Nucleohiston. Ihid., 1900, xxx, p. 508. 



Raises the question as to the nature of Lilienfeld's nucleohiston. 



4. . Reply to Kossei. Ibid., 1901, xxxi, p. 407. 



5. . Reply to Kossei. Ibid., 1901, xxxii, p. 79. 



6. . Chemische Untersuchungen der lymphatischen Organe. Bei- 

 träge mir Chem. Physiol. u. PathoL, 1904, v, p. 317; iv, pp. 115, 

 351. Füll report of Bang's experiments, with review of the work 

 of Malengrau and Huiskamp. 



7. Eddy, W. H. A study of some protein Compounds. Biochemical 



Bulletin, 1912, ii, p. iii. Reported attempts to form protein 

 salts with histon and difficulties in preparing acid-free histon. 



8. Ehrström, Robert. Ueber ein neues Histon aus Fischsperma. 



Zeitschr. f. physiol. Ch., 1901, xxxii, p. 350. Preparation of Lota 

 histon from the sperm of the burbot. 



9. Fleroff , A. Ueber einen histonähnlichen Körper aus Thymus. Ibid., 



1899, xxviii, p. 307. Discovery and preparation of para-histon 

 from thymus ; comment on water-insolubility of ammonia-precipi- 

 tated histon. 



