460 Proceedings Colnmhia Biochemkal Association [April 



thigh, the mtiscles snpplied by tlie cut nerve do not take part in the 

 subsequent convulsions. Successive destruction of the cerebrum, 

 optic lobe, and medulla does not prevent the development of the 

 convulsions nor does it modify them after they have appeared. 

 Destruction of the cephalic half of the cord prevents the tetanus of 

 the arms but does not afifect the spasm of the legs. Total destruc- 

 tion of the cord permanently abolishes the convulsions. 



A study of the afferent nerves shows that the Stimulus must be 

 fairly abrupt, since dilute acid may be applied without inducing any 

 reaction while the application of strong acid is regularly followed 

 bv convulsions. 



Cocainizing an area of the skin lessens the tendency to convul- 

 sions from irritation of that particular area. Provided the Stimula- 

 tion involves the skin only, no convulsion usually results. If the 

 Stimulus be a little stronger, so as to cause pressure on a tendon or 

 movement of a Joint, a convulsion ensues. If a sensory nerve be 

 cut, and the area of skin supplied by that nerve be stimulated, no 

 reflex or convulsion occurs. Stimulation of the central end of the 

 divided nerve is followed by typical tetanus. 



The convulsions, then, are of reflex origin, and apparently can 

 be best explained by assuming that potassium sulfocyanate causes 

 changes in the cord resembling those induced by strychnine. I have 

 repeatedly attempted to perform experiments similar to those of 

 Baglioni but have never succeeded in exposing the cord without 

 destroying the reflexes. 



72. Experimental agglutinative and hemolytic trans- 

 fusions.^^ R. Ottenberg, D. J. Kaliski and S. S. Friedman. 

 By a suitable technic, iso-agglutination and iso-hemolysis can be 

 demonstrated to occur between the bloods of different dogs. Iso- 

 agglutinins occur naturally, and it is possible that the immune iso- 

 agglutinins produced by von Dungern and Hirschfeld are merely 

 intensifications of these. No sharp grouping (such as would indi- 

 cate a limited number of agglutinable substances and of agglutinins) 

 could be made out, however, in the naturally-occurring agglutinins. 



" Under the auspices of the George Crocker Special Research Fund. Some 

 of this work was done in the Pathological Laboratory of Mt. Sinai Hospital. 

 The authors wish to thank Dr. W. Thalheimer for his assistance with the 

 histological part of the work. Jour. of Med. Research (in press). 



