4^4 Proceedings Coliimhia Biochemical Association [April 



75. A simple classroom experiment for demonstrating the 

 production of acid by contracting muscle. E. N. Harvey and 

 W. E. HoY. {Physiologicol Lahoratory, Princeton University.) 

 The experiment is based on the fact that ammonium hydroxid 

 readily penetrates living tissties and hence may be used to neutralize 

 the acid produced in muscle cells during functional activity. The 

 skinned legs of a frog are stained in neutral red and one is electric- 

 ally stimulated. The stimulated leg becames slightly more red 

 but the difference is not readily detected by Student eyes. Both legs 

 are then placed in physiological salt Solution containing n/200 am- 

 monium hydroxid. The unstimulated muscles are immediately 

 turned yellow by the ammonium hydroxid while the stimulated 

 muscles retain their red color. The experiment can be performed 

 in a very short time, the color change is striking and the reaction 

 a delicate one. The acid produced by ten induced shocks may easily 

 be detected under the proper conditions. 



76. The relation of acapnia to shock, and a consideration of 

 the mechanical effects of hyper-artificial respiration upon the 

 circulation. Henry H. Janeway and Ephraim M. Ewing. 

 (Laboratories of Experimental Siirgcry and Physiology of the New 

 York University and Bellevue Hospital Medical School, N. Y.) 

 Published in füll in this issue of the Biochemical Bulletin^ page 



403. 



77. Metabolism in a case of myotonia atrophica. Jacob 

 Rosenbloom and Benson A. Cohoe. {St. Francis Hospital and 

 Lahoratory of Biochemistry of the University of Pittshurgh, Pitts- 

 biirgh, Pa.) In a thirteen day metabolism research on an individual 

 suffering from myotonia atrophica, we have determined the nitrogen 

 metabolism and urinary nitrogen partition, the sulfur metabolism 

 and urinary sulfur partition, also the calcium, magnesium, phos- 

 phorus, chlorin and fat metabolism. The only striking metabolic 

 anomaly was marked loss of calcium. The Creatinine excretion was 

 normal. 



78. A method for the determination of tryptophan in pro- 

 tein material. Jesse A. Sanders and Clarence E. May. 

 (Chemical Laboratories, University of Indiana, Bloomington, Indi- 

 ana.) Published in füll in this issue of the Biochemical Bul- 

 letin, page 373. 



