466 Proceedings Columbia Biochemical Association [April 



as were the Clitocybe and Inocyhe products. This tends to con- 

 firm the observations of others that muscarin is not the only poison 

 in A. muscaria. 



The edible Clitocybe multiceps yields no toxic material when 

 treated in the same manner as these poisonous fungi, showing that 

 oiir manipulations were not responsible for the effects observed. 

 The ash constituents of the poisonous fungi were found to have 

 no efifect on frogs. 



From our studies on Inocybe infida and Clitocybe illiidens, and 

 from Ford's work upon the latter and Inocybe infelix, it is piain 

 that these plants should not be eaten, for they contain toxic material 

 not unlike muscarin. 



81. The phosphorus content of the blood and serum of 

 normal and parathyroidectomized dogs.^''^ Isidor Greenwald. 

 After parathyroidectomy the amount of phosphorus in the blood and 

 serum is increased. The increase is chiefly in that fomi of phos- 

 phorus that may be extracted with dilute hydrochloric acid Solu- 

 tion containing picric acid to prevent swelling of the protein. 



82. Further metabolism experiments upon parathyroidec- 

 tomized dogs.^^ IsiDOR Greenwald. The retention of phos- 

 phorus after parathyroidectomy is followed or accompanied, but 

 not preceded, by a retention of sodium and potassium. 



83. A study of uroerythrin, with demonstrations. Beatrix 

 H. Gross. In the last edition of his mimeographed directions for 

 laboratory work in physiological chemistry, Dr. Gies describes, as 

 follows, a method for the extraction of urochrome from urine.^^ 



Treat about 25 c.c. of urine with phenol, little by little, with thoro 

 stirring until the liquid remains decidedly turbid. The pigment is not 

 affected by the phenol. After saturating the urine with phenol in this 

 careful manner, add about i c.c. of phenol in excess and then saturate 

 the liquid with ammonium sulfate. As the ammonium sulfate dis- 

 solves, the phenol is rendered insoluble. The yellowish turbidity is due 

 to emulsified phenol, which carries urochrome in Solution. The yellow 



" Some of the work was done in the Pathological Laboratory of Columbia 

 University and the Chemical Laboratory of the Montefiore Home, New York. 

 Journal of Biological Chemistry, 1913, xiv, p. 369. 



^ See foot note 17. Ibid., p. 363. 



"This method is based on the findings of Kramm: Deutsche medizinische 

 Wochenschrift, 1896, xxv, p. 42. 



