550 Proceedings Columbia Biochemical Association [July 



mold: (a) Very rapidly — auramin, bismarck brown, chrysoidin, 

 erythrosin, martius yellow, metanil yellow, orange G, rose bengal; 

 {h) in fifteen minutes — eosin A, phloxin, safranin; (c) in one hour 

 — Biebrich scarlet; (d) in one day — cape aloes, fast red A, fustic 

 extract, rhodamin ; (e) indiffusihle — benzopurpurin, malachite green, 

 methylene blue. The following dyes (those named above) diffuse 

 through coUodion-fat membranes used immediateiy affer removal 

 from the mold: (a) Very rapidly — chrysoidin, eosin A, martius yel- 

 low, orange G, phloxin, safranin; {b) in fifteen minutes — erythro- 

 sin; (c) in thirty minutes — Biebrich scarlet, metanil yellow, rose 

 bengal; {d) in one day — auramin, cape aloes, fast red A, rhodamin; 

 {e) indiffusible — azolitmin, benzopurpurin, bismarck brown, mala- 

 chite green, methylene blue. 



The older the bags of each kind, that is the more completely the 

 residual ether and alcohol solvents of the collodion Solution (U. S. 

 P. ) were removed from the membranes, the less permeable the bags 

 became. In most cases, each particular pigment diffused through 

 the different membranes at the same general rate, but in the follow- 

 ing instances diffusion through collodion-fat membranes was faster 

 or slower than through piain collodion membranes: (a) faster — 

 Biebrich scarlet, eosin A, phloxin, safranin; {b) slower — auramin, 

 bismarck brown, erythrosin, metanil yellow, rose bengal. 



Certain variations have been noted which appear to depend on 

 irregularities in the dyes and in commercial U. S. P. collodion Solu- 

 tions, also on the " age " of the bags. The study is in progress. 



97. Further studies of edema: On the absorption of water 

 by white lupin seeds. Tula L. Harkey. The swelling of 

 weighed, normal, white lupin seeds in various media, as compared 

 with the increase in weight of such seeds in distilled water, was 

 determined at short intervals for a week or more. At the end of 

 24 hours, seeds gained more weight in the chemical Solutions than in 

 the corresponding water controls, in the instances cited on the 

 opposite page. 



Effects of other acids, and of electrolytes and non-electrolytes 

 on the imbibition of water from acid Solutions, will be reported 

 later. 



98. Further studies of edema: On the postmortem absorp- 

 tion of water by tissues from well nourished and f asting animals. 



