1913] Alfred P. Lothrop 555 



103. A study of the influence of extemal hemorrhages on 

 the partition o£ urinary nitrogen. Olive G. Patterson. Two 

 dogs were subjected to external hemorrhages (3.5-5.8 per cent. of 

 body weight) under local anesthesia (cocain), and studied by some 

 of the nutritional methods in use in this laboratory. One dog was 

 subjected to four successive bleedings, at intervals of 11, 5 and 7 

 days, respectively. The general conclusions pubHshed by Hawk 

 and Gies/'^ on the effects of external hemorrhage in dogs under 

 general anesthesia, were confirmed. Each hemorrhage caused abso- 

 lute increases in the renal excretion of total nitrogen and urea. The 

 first hemorrhage in each animal was followed by an increased Out- 

 put of Creatinin; subsequent bleedings increased the excretion of 

 Creatinin or were without influence on it. The absolute amounts 

 of urinary ammonia, uric acid and purins were unaffected by the 

 hemorrhages. The results will be published in detail in the near 

 future. 



104. The action of a high frequency current on the activity 

 of pancreatic amylase. P. W. Punnett. An attempt was made 

 to discover what effect, if any, the high frequency current, such as 

 is used in electro-therapeutics, has on the activity of pancreatic 

 amylase. The current was furnished by a high frequency machine 

 loaned to the department by the Van Houten and Ten Broeck Com- 

 pany of this city, to whom we are greatly indebted for this courtesy. 

 The amylase of commercial "pancreatin" (Parke, Davis & Co.) 

 was used. The method of Sherman, Kendall and Clark^^ was 

 employed throughout for the determination of activity. 



Solutions of the material were treated with the current in a 



beaker coated on the outside with tinfoil attached to one terminal 



of the machine and having a platinum electrode, connected to the 



other terminal, immersed in the Solution, the whole being kept in an 



ice bath. The length of treatment varied from 5 to 30 min., and 



the hot-wire ammeter gave a reading of 500 to 600 milliamperes. 



Controls were kept at the same temperature. Because of the fact 



that only ordinary distilled water was available, the enzyme did not 



show more than 70 per cent. of its maximum activity (its power was 



418, using triple-distilled water). 



'^'' Hawk and Gies : Amer. Jour. of Physiol., 1904, xi, p. 171. 

 18 Sherman, Kendall and Clark: Jour. Amer. Chem. Soc, 1910, xxxii, 

 p. 1073. 



