THE EUGLENOIDINA OF OHIO 345 



(b) METHODS. 



The apparatus and supplies needed in a study such as out- 

 lined consist of a good microscope provided with an oil immersion 

 objective and the general accessories. The instrument will cost 

 approximately $75 and a Spencer 40 or 46 G, or a Bausch and 

 Lomb BB-8 will be found quite satisfactory, although where cost is 

 not a prohibitive factor the Leitz "Monobjective" binocular, cost- 

 ing approximately $250, duty free, with the apochromatic optical 

 equipment is to be recommended. Magnifications should range 

 from 50-2500. Among accessories may be mentioned slides ; cover 

 glasses (22-25m round); half dozen small pipettes; one dozen 

 watch glasses (Syracuse pattern) ; a stage micrometer ruled in 

 1/10 and 1/lOOmm; an ocular micrometer; camera lucida ($10- 

 $20) ; 2-H and 6-H "Kohinoor" drawing pencils; lens paper; dis- 

 secting needles, tweezers, and scissors, etc. An Irving Pitt No. 

 9108 Note Cover (I-P Mfg. Co., Kansas City), with paper punched 

 to fit (procure a light weight bond at a paper supply house, size 

 8 X 10V1>. and have punched to fit cover) is useful in keeping notes 

 and drawings together. These supplies, with the exception of 

 the Note Cover, may be procured from the Spencer Lens Co., of 

 Buffalo ; the Bausch and Lomb Co., of Rochester, or from the 

 U. S. branch of E. Leitz, 30 E. 18th Street, New York City. 



Small drinking glasses with rectangular pieces of glass placed 

 on top to prevent too rapid evaporation (a slight opening should 

 be left) , may be used as aquaria, while one or two quart milk pails 

 make excellent collecting receptacles. Where smaller quantities 

 of material are collected, and it is desirable to prevent contamina- 

 tion, ground glass stoppered bottles with metal screw caps (Betz 

 Co., Hammond, Indiana), which come in pads usually containing 

 six bottles, may be used. These are easily sterilized and may be 

 kept as small aquaria until the culture is exhausted. Quantitative 

 methods of study have not been utilized in the present paper. 



(c) NEW SPECIES. 



Four new species of Euglenoidina are noted in the following 

 pages. Euglena simylacra from Fremont, Ohio. Eugloia tnnicata 

 from Hiawatha Lake, Mt. Vernon, Ohio. Scj/tonwnas dobelli from 

 the digestive tract of Molge rul(/ar[!^, Europe. Plocofia mrwiua, a 

 marine form, from Woods Hole, Mass. 



