11 



the rest by means of a hypodermic syringe, the results being the same, 

 except that the symptoms appeared sooner when a large nnmber of 

 germs were inserted. All of the inocnlations were made in the middle 

 or terminal parts of healthy leaves, with one exception, in which case 

 the germs were inserted into the upper part of a flower shaft before 

 the buds opened. 



Much to my surprise, the progress of the disease was very slow, 

 exactlj^ as described by Dr. Wakker, and the striping down of the 

 disease was restricted in most cases to long, narrow areas, with healthy 

 green tissue to either side. In case of the hypodermic injections, 

 however, a width of three to eight or more vascular bundles was 

 involved, i. e., as much breadth of tissue as appeared water-soaked 

 after the injection, but not much more. Even when a great quantity 

 of germs Avas injected (0.5 cc. or more of a fluid culture) the disease 

 did not appear immediatel}^, develop rapidly, or cause widespread 

 infection of the bulbs. 



To show how closely my results tally with those obtained by Dr. 

 Wakker,^ I will here set down the course of the disease in each of the 

 eight plants first inoculated. 



' See Contributions k la pathologie vegetale, I, La maladie du jaune, ou maladie 

 nouvelle des jacinthes, causes par le Bacterium Hyaciiithi, Archives neerlandaises 

 des sci. ex. et naturelles. Tome XXIII, pp. 18-20. 



