27 



tiires being made. One of these scapes received 0.3 cc. of the cloudy 

 brotli and the other 0.6 ec. 



Result. — Four of the six inoculated leaves showed distinct symp- 

 toms on February 14. No stripes were visible on the other two until 

 after February 17, and they were slight on March 1, consisting merelj^ 

 of some narrow, parallel, water-soaked lines. On March 1 two of 

 the other four leaves were shriveled to the base, and a third was 

 shriveled halfway down and showed water-soaked places farther 

 down. Neither of the plants inoculated in the scape showed any 

 sjnnptoms until after February 17, all of the flowers wilting normally. 

 On March 1 the scape which received 0.3 cc. showed one very narrow, 

 short, water-soaked stripe in the npper i^art under the shriveled flow- 

 ers, and at the end of this niontli some of the leaves began to be 

 3^ellowish-green between the vascular bundles as if disturbed in their 

 nutrition. The scape which received 0.(5 cc. showed on March 1 two 

 or three narrow, short, water-soaked lines below the shriveled flow- 

 ers. At the end of this month the scape was wholly shriveled and 

 the leaves dead at the top (upper 3 to 6 cm.). On June 17, when the 

 bulbs w^ere dug for examination, all were spoiled by the soft rot. 



The leaves of the check i)lants never developed any symptoms of 

 the yellow disease. On June 17, when the bull)s were dug for exam- 

 ination, all of them were soft-rotted at the heart, but none of them 

 showed any trace of yellow bundles. 



SEmES 11 (CABBAGE). 



The eleventh series of inoculations was made on young cabbage 

 plants in active growth. They were inoculated Februarj^ 11 from the 

 same culture as the preceding (No. G, February' 5). On each of two 

 plants the germs were forced into several parts of two leaf blades by 

 means of the syringe, and on each of the same leaf blades numerous 

 delicate punctures were made with the tip of the needle and the fluid 

 bearing the germs was carefully rubbed in and not allowed to dry 

 immediately. To prevent any injurious action of sunshine or of dry 

 air large drops of the culture were Anally put on the punctured parts 

 and sheltered from the direct action of the light and of air currents 

 until nightfall. The germ-laden fluid was forced into 2 petioles of a 

 third plant, so that they showed long, suffused streaks, while here and 

 there the fluid oozed through the epidermis in many very tiny drops. 

 The blade of a third leaf on this plant was punctured, inoculated, 

 rubbed, covered with fluid, and sheltered as described above. 



Result. — After some days the two injected petioles split open, but 

 no otlier symptoms appeared, not even in the immediate vicinity of 

 the injected and punctured parts. The plants were under observ^a- 

 tion nearly four months, and differed in no respect from the check 

 plants. Inoculations of such plants with Pseudonionas cnmpestris 

 led to very different results, as I have shown elsewhere.^ 



'See Centralb. f. Bakt., 2. Abt. Bd. Ill, July, 1897, p. 284. 



