28 



SERIES 12 (AMARYLLIS). 



The twelfth series of inoculations was made February 11 on the 

 well-grown leaves of AinarylUs atamasco. Two plants, not j^et show- 

 ing any flower shoots, were selected for this purpose, and three healthy 

 plants in the same pot were held as checks. Two leaves were selected 

 on each plant and 0.3 cc. of the cloudy broth culture (No. 6, February 

 3) was injected into each of two places on each leaf. 



Result. — The sjnuptoms developed verj^ slowly as feeble yellowish 

 stripes, confined to the parts originally suffused. Subsequently in the 

 striped part the plants produced, as is their wont when injured, a red 

 pigment. On March 1 this red pigmentation was quite distinct in each 

 one of the eight inoculations. On March 31 it was more pronounced, 

 occurring mostly in the form of interrupted red streaks on a green 

 background. These were visible on one leaf for 10 cm. below one of 

 the needle punctures and for 12 cm. above the other. On another 

 leaf the red dots and stripes extended 7 cm. above a needle puncture 

 and 9 cm. below it. At this point there were more than 100 red dots, 

 each less than 0.5 mm. in diameter. These red spots were in parallel 

 rows over vascular bundles and not in the parenchyma between the 

 bundles. In the widest part of the stripe four vascular bundles had 

 these red spots over them. In the oldest and worst stained part of the 

 stripe (near the puncture) the red stain also involved the parenchyma 

 between the bundles. After this date the disease made only very slow 

 progress. On June 18 the bulbs were knocked out of the pot, sec- 

 tioned at many levels, and carefully examined. All were entirely 

 free from any trace of yellow bundles and j)erfectly sound. 



SERIES 13 (HYACINTHS). 



The thirteenth series of inoculations was undertaken February 12, 3 

 p. m., to determine whether infections might not be secured through 

 the blossoms. For this purpose I selected four single, blue-flowered, 

 healthy i)lants of Baron van Tuyll, four plants of the same variety 

 and in the same pot being held as checks. All were in full bloom. Six 

 flowers on each of the four plants were inoculated by putting a big 

 drop of cloudy beef broth (No. 11, February 3) gently into the throat 

 of each one without in any way touching the flower wi^h the needle 

 of the hypodermic syringe. The pot and the earth on which it stood 

 were heavih' watered and then covered with a large bell jar. This jar 

 was removed February 14, at noon, when the drops had disappeared. 

 Bees had access to the hothouse and visited these plants freely all 

 day, but for the most part they carefully avoided the inoculated 

 flowers. In one instance, however, I saw a bee enter an inoculated 

 flower. Frequently they passed in front of such flowers and occa- 

 sionally prepared to enter and then suddenly withdrew and selected 

 uninoculated flowers. 



The throat of the contracted perianth did not wet readily, and so 



