30 



dug and examined, the three check plants were entirely sound. Three 

 of the inoculated plants were also sound, or at least appeared so to 

 the unaided eye. The bulbs of the two other plants were sound 

 externally, but on sectioning them they showed unmistakable symp- 

 toms of the yellow disease. One was slightly affected in two scales. 

 The other was more seriously diseased, as will be seen from the fol- 

 lowing account of it: 



Notes on plant No. 67. — February 12, 3 p. m. Inoculated six flowers. 



February 14, noon. Removed the bell jar. The heart of the inoculated flowers 

 is still moist. 



February IT. The flowers begin to shrivel. The inoculated ones are holding 

 up best. 



March '2. The flowers have withered. The scape is large and 80 cm. long. 

 Its upper 2 cm. is yellow and shriveling, but there are no symptoms attributable 

 to the inoculations. The rest of the scape is green and turgid. The leaves are 20 

 cm. long and are healthy. 



March 81. The scape has dry-shriveled and all of the leaves are drying out at 

 the tip (3 to 10 cm. j. The plant looks bad, but there are no stripes on the leaves, 

 not even at their extreme base. 



June 17. Leaves dead, bulb sound externally. On cutting, twenty-two yellow 

 bundles were found in the upper part of the white and otherwise sound plateau. 

 The infected bundles were all on one side of the bulb and were beautifully distinct, 

 as in case of No. 63. In the upper part of the bulb eleven bundles in four scales 

 were visibly affected, the j'ellow slime oozing from the cut surface. Lower down 

 (near the plateau) a larger number of bundles were yellow, and one other scale 

 was involved (one bundle, in which the yellow disappeared about halfwaj- up). 

 The extreme base of the plateau was sound, and. as in No. 63, the progi-ess of the 

 infection was clearly from the scape to the vessels of the plateau and from the 

 latter to the scales. There was no soft white rot. 



SERIES 15 (ONIONS). 



The fifteenth series of inoculations was made February 12 on Allium 

 cepa. Four well-grown onion plants not yet in bloom were selected 

 for this purpose. The inoculations were by means of a hypodermic 

 sja'inge, using the well-clouded beef broth in tube Xo. 11 (February 4). 

 About 2 cc. was injected into one plant, numerous punctures being 

 made into old and young leaves. Three leaves were selected on each 

 of two other plants and 0. 3 cc. was injected into the base of each one. 

 Ilie fourth plant was inoculated in the same way, 0.3 cc. being injected 

 into the base of each of four leaves. 



Result. — On March 2 the inoculated leaves, in whole or in part, Avere 

 shriveled and white. On March 31 there were no additional symptoms. 

 On June 18, when the bulbs were dug and sectioned, all were free 

 from yellow bundles and entirely sound. 



SERIES 16 (hyacinths). 



The sixteenth series of inoculations was made February 16, at 11 a. m. 

 Two single, white-flowered hj acinths, of the variety known as Baron 

 van Tuyll, were selected for this purpose and two plants of the same 



