36 



No spores could he found in ji culture which had grown in the ice 

 chest for 50 days. 



A culture at room temperatures w^as feebly alkaline to neutral litmus 

 after 50 da^-s. A culture which had been kept in the ice chest for the 

 same lenotli of time, and consecuiently was not so far advanced in 

 growth, was distinctly alkaline, i. c., more so than the preceding. 

 After Si days in the ice chest a culture was strongly alkaline to neutral 

 litmus. 



No acid reaction was observed. 



No brown pigment was developed. After -19 days at room temper- 

 atures the substratimi was as white as when inoculated. 



No cy^tohydrol3'tic action was observed. After the organism had 

 grown on it for 81 days (ice chest) the substratum was as tough as 

 when iirst inoculated. 



No cr3^stals were observed and there was no decided smell. 



Ps. .sfewartl grew in nmch the same waj^ on this substratum, but 

 frequenth" made less growth. The color of its slime was buff j^ellow, 

 and cr3\stals were formed. 



Radish. 



Slices of small, tender, red-skinned, turnip-rooted radishes were pre- 

 pared in the same way as the potato. 



On this substratmn the hyacinth organi^^m made a good growth, as 

 the followino- record shows: 



Stock 211. — ]Mufh water. Inoculated February 19, 5 ]>. iii., from a lu'cf-broth cul- 

 ture 14 days old, and kept at room temperatures. 



February 22, 3 p. in. A good growth on the surface above the water, pale yellow, 

 wet-shining. Fluid clouded, no precipitate. 



February 26. A wet-shining, pale yellow growth over the whole exposed surface. 

 A good grf)wth, Init not more copious than that in a corresponding tube of Ps. cam- 

 pestris. A moderate amount of precipitate. This is a yellower germ than J's. nnn- 

 pestris. It shows so, plainly, on all four media (radish, turni}), carrot, and coconut). 



March 5. A copious growth. No l)rown pigment. 



March 16. No brown stain. 



April 9. The culture has begun to dry out, Init there is still about one-half c. c. of 

 fluid in the bottom of the tube. There is a thin pale-yellow precipitate. The sub- 

 stratum has changed color decidedly. The check tubes are still white, l)ut the sub- 

 stratum in this one is of a color not easily described, i. e., unlike any in Ridgway's 

 color system. It approaches his raw Sienna ( V-2) , and if that color had in it a very 

 slight amount of brown it would closely resemble the color of this substratum. On 

 long standing, therefore, a brownish pigment appears in tubes of radish. 



A year later this experiment was repeated, using globose red and 

 oblong white radishes. The results were substantiallv the same. 

 There was a copious, very wet-shining, very pale-yellow growth, which 

 never became ])right yellow like that on coconut. In each case the 

 substratum tinally became brown, but this change took place very 

 slowly, and the color never became deeper than a pale russet (64 days). 



