42 



The color on the fifth day was " bright yellow." On the eighth day 

 it was between chrome yellow and maize yellow (R. VI-8 and 21). 

 These two colors are compounded of varying amounts of orange cad- 

 mium, pale cadmium, and white. The slime in old cultures became 

 dark(U', as if from admixture with a brown stain. On the thirty -fir.st 

 day the color was between ochraceous and raw sienna (R. V-2), being 

 near the latter color. After sixty-nine days the slime in one tube was 

 noted as "dark yellow" and in another as ochraceous to tawny ochra- 

 ceous. In one of these tubes the carrot was observed to be decidedly 

 deeper orange than when it was inoculated; i. e., than check tubes. 

 On the fourteenth day the color and general appearance of this slims 

 closely resembled that of a culture of Ps.jjhnseoli made for comparison. 



On the eighth and twenty-third days the slime was distinctly alka- 

 line to neutral litmus paper. On the same da}^ the slime was more 

 alkaline in a tube 31 days old than in one of the same age and origin, 

 but in which the organism had grown for onl}' 23 days; i. e., was 

 restrained from growth by heat during the first week. In a culture 

 67 daj'S old the slime was plainly alkaline. 



After two and one-half months'' growth, the carrot cylinders retained 

 their form perfectly (2 tubes), but went into pulp easily under pres- 

 sure of the fingers, as if the middle lamella had been partially dis- 

 solved. These cylinders had a soapy-feeling, and a feeble but distinct 

 smell suggestive of ammonia and amin compounds. 



In one instance crystals or crj^stal-like bodies were observed in the 

 slime of old cultures (09 days). 



Penicillium grew readily on carrot covered by this organism, was 

 found associated with it in a number of the l)ulbs received from the 

 Netherlands, and is mentioned by Dr. Wakker as sometimes occurring 

 in badly affected Indbs. 



A repetition of the carrot cultures in 1899 led to similar results. 



In two test-tube cidtures which were examined after seventy-two 

 days the growth appeared to be tj^pical for Ps. hyacinthi^ but in one 

 the carrot was browned and in the other not. In both cultures there 

 was a feeble smell, like glue; in both the cylinders were softened and 

 went to pieces under slight pressure of the fingers. In the one which 

 was not browned the carrot was distinctl}" but feebh' acid to neutral 

 litmus paper (it was also acid on the fortj'-second day). In the other 

 the surface slime was neutral to litmus (it was alkaline on the fortj"- 

 second day). The interior of the carrot was also neutral or nearly so. 

 Lead acetate- paper placed for six weeks in the mouth of this tube, 

 below the cotton plug, was not browned. The C3dinders are believed 

 to have been derived from different carrots. Both cultures were 

 inoculated from the same tube. The difference in brown staining is 

 believed to be attributable to slight differences in the chemical com- 

 position of the carrots. (See Ps. phaseoli under The Brown Pigment.) 



